Research development of CRISPR/Cas9 system on parasitic studies

Abstract

Abstract:

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats)/(CRISPR-associated protein 9) is an efficient genome editing system for targeted gene disruption, site-specific insertion of foreign DNA, gene repair, etc., which provides an innovation platform of technology for functional analysis of parasite genes and selection of drug targets and vaccine candidates. Here, we review the mechanism of CRISPR/Cas9 and some recent findings on the use of CRISPR/Cas9 in parasite research, particularly in studies of Toxoplasma gondii, Plasmodium, Trypanosoma and Leishmania. The challenges and optimization strategies of the CRISPR/Cas9 system at the present stage are also discussed.

Key words: CRISPR/Cas9 system, Gene editing, Parasite

CLC Number:

Yi-xiu FU, Qing-ming KONG, Shao-hong LU. Research development of CRISPR/Cas9 system on parasitic studies[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(3): 299-304.

References 54

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