›› 2013, Vol. 31 ›› Issue (5): 19-410-411.

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Genotypes of Toxoplasma gondii Isolates from HIV Positive Patients in Yunnan Province

NIE Da-ping1，2，JIA Yu-xi1，CHEN Ling-juan1，YOU Ying-xia1，LI Wei1，SHEN Li-jie3 *   

  1. 1 Department of Parasitology，College of Basic Medicine，Dali University，Dali 671003，China；2 Ji’an County People’s Hospital of Jiangxi Province，Ji’an 343100，China；3 Department of Pathogen Biology and Immunology，Kunming Medical University，Kunming 650500，China
  • Online:2013-10-30 Published:2014-07-24

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Abstract:  One hundred and fifty serum samples of HIV positive patients were collected in western Yunnan Province from September 2011 to December 2012. Toxoplasma gondii B1 gene was amplified by nested PCR. Genotyping of T. gondii isolates were performed by restriction fragment length polymorphism（RFLP） with Pm1Ⅰ and XhoⅠ. 13 samples were found positive with the B1 gene （530 bp） amplification and belonged to type Ⅰ. The sequencing results showed that 4 T. gondii B1 gene positive samples were identical, with 3 nucleotide variation compared with T. gondii strain RH （type Ⅰ） B1 gene （GenBank No. AF179871）, and in the other sample a “G→A” mutation at 230 bp was detected. The results indicated that the genotype of Toxoplasma gondii in HIV positive patients in Yunnan Province is type Ⅰ.

Key words: HIV, Toxoplasma gondii, B1 gene, Nested PCR, Genotyping