›› 2013, Vol. 31 ›› Issue (2): 20-148-150.

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Sequence Homology Analysis on Lactate Dehydrogenase(LDH)of Plasmodium vivax Anhui Isolates

HU Ming-jie1,FANG Qiang2 *,WU Shou-wei1,TANG Bi-kui1,ZHANG Jing1,JIAO Yu-meng2,XIA Hui2,SHEN Ji-long3   

  1. 1 Department of Gentics,Bengbu Medical College,Bengbu 233030,China;2 Department of Microbiology and Parasitology,Bengbu Medical College;Anhui Key Laboratory of Infection and Immunity,Bengbu 233030,China;3 Department of Microbiology and Parasitology,Anhui Medical University,Hefei 230032,China
  • Online:2013-04-30 Published:2013-07-02

Abstract:  DNA in dried blood spots of 39 vivax malaria patients (2009-2010) from Anhui(Bengbu urban district and counties of Wuhe, Huaiyuan, Mengcheng and Lixin) was extracted. The Plasmodium vivax LDH(PvLDH) gene was amplified, cloned and sequenced. The sequences were subjected to NCBI Blast program. The results showed that the targeted DNA fragment size was 951 bp without difference among the 39 samples(accession No. GU078391), and was more than 99% homologous to the PvLDH sequences in other strains from GenBank. There was only one different amino acid in the protein sequences between the isolates from Anhui and EJEU60134 or MIA061251 strains.

Key words: Plasmodium vivax, Lactate dehydrogenase, Homology analysis