Objective To sequence and analyze the complete nucleotide sequence of the mitochondrial genome of Oncomelania hupensis. Methods Four long fragments were amplified by long PCR using the primers designed based on mtDNA-COⅠ， Cytb， 16S rRNA and COⅢ gene sequences， and sequenced by conserved primer-walking. Rusults The mitochondrial genome （GenBank accession no. FJ997214） was a circular molecule of 15 182 bp with a total A+T content of 67.32%， and contained 13 protein-coding genes， 2 ribosomal RNA genes， 22 tRNA genes， and an A+T-rich region of 72 bp. All 13 protein-coding genes of the O. hupensis mtDNA used ATG as start codon. Canonical TAA and TAG termination codons were found in 12 protein-coding genes， and the remaining one （ND1） had an incomplete termination codon （T）. Two short gene overlaps were found with a length of 4 bp and 7 bp， respectively. The length of 21 total intergenic region of mtDNA was 145 bp ranging from 1-30 bp. A total of 22 transferring RNA were found， all of which were typical cloverleaf structure except for two tRNASer， one tRNAGln and one tRNAIle. Conclusion The complete sequence of O. hupensis mitochondrial genome has been determined.