›› 2009, Vol. 27 ›› Issue (3): 4-209.

• 论著 • Previous Articles     Next Articles

Transcription Profile of PfRON4 Gene in Plasmodium falciparum Erythrocytic Stage

CAO Jun1, 2, Osamu KANEKO3, GAO Qi1 *, ZHOU Hua-yun1, XIA Cao-ming1, ZHUGE Hong-xiang1, Takafumi   

  1. 1 Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China；2 Department of Pre-clinical Medicine, School of Medicine, Suzhou Unversity, Suzhou 215123, China；3 Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan；4 Cell-Free Science and Technology Research Center, Ehime University, Ehime 790-8577, Japan； 5 Department of Molecular Parasitology, Ehime University, Ehime 791-0295, Japan
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30
  • Contact: GAO Qi1

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Abstract: Objective To reveal the transcription profile of PfRON4 gene in Plasmodium falciparum erythrocytic stage. Methods P. falciparum schizonts were purified by differential centrifugation on a Percoll-sorbitol gradient, after which the released merozoites were allowed to invade uninfected erythrocytes for 4 hours before the clearance of all remaining schizonts using 5% D-sorbitol. The cultured synchronous parasites were harvested for RNA assay immediately, 24 hours later, and then at every 6th hour. PfRON4 and related genes (PfAMA1 and PfRhopH2) were amplified by real-time PCR for establishing standard curves to evaluate the copy number of genes. Results P. falciparum parasites were well sychronized. Those quantative analyses were reliable because the R value of standard curves were more than 0.98 and the melting curve showed a single peak. When parasites were in the schizont stage, PfRON4 gene transcription reached a peak in 36-40 hours after invasion. Conclusion The transcription of PfRON4 peaks at mature schizont stage, suggesting that the PfRON4 gene may involve in erythrocyte-invasion of P. falciparum.

Key words: Plasmodium falciparum, PfRON4, Real-time PCR, Transcription