Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting

›› 2008, Vol. 26 ›› Issue (4): 17-320.

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Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting

LI Lin-jie^1,2, BAO Huai-en^{1 *}, PAN Wei³

1 Department of Parasitology, Guiyang Medical College, Guiyang 550004, China; 2 Department of Medical Laboratory, Guiyang Nursing Vocational College, Guiyang 550004, China; 3 Department of Medical Laboratory, Guiyang Medical College, Guiyang 550004, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-08-30 Published:2008-08-30

Abstract

Abstract: The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis （2-DE）, and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels （7×8 cm, pH 3-10）． Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.

Key words: Toxoplasma gondii, Tachyzoite, SD rat, Antigen, Two-dimensional electrophoresis

LILin-jie;BAOHuai-en*;PANWei. Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting[J]. , 2008, 26(4): 17-320.

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Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting

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