Abstract: Objective To clone and express a novel protein analogous to TIP（T cell immunomodulatory protein）of Plasmodium berghei ANKA and prepare its polyclonal antibody. Methods The PbTIP encoding nucleotide sequence was searched from the Plasmodium berghei genomic database and amplified by PCR. The gene was sub-cloned into prokaryotic expression vector pGEX4T-1 and expressed in E.coli BL2l （DE3）. After induction by IPTG， the expression of PbTIP-GST fusion protein was characterized by SDS-PAGE and Western blotting. The inclusion bodies of GST-PbTIP fusion protein were injected into BALB/c mouse. Anti-sera were identified by indirect fluorescent antibody test and western blotting. Results The PbTIP-GST fusion protein was successfully expressed in the form of inclusion bodies， by controlling the temperature and concentration of IPTG. Anti-PbTIP-GST sera were acquired with high titer. The sera specifically recognized the PbTIP with a band of 60 000 in P.berghei infected erythrocyte protein. Conclusion PbTIP/GST fusion protein and polyclonal antibody have been obtained.

Key words: TIP, Plasmodium berghei, Prokaryotic expression