Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules

doi:10.12140/j.issn.1000-7423.2020.02.021

Abstract

Abstract:

To investigate the affects of Plasmodium berghei infection on spleen B cells and NK cells as well as their surface molecules in mice, 10 female C57BL/6 mice were randomly assigned into control group and infection group, five mice each. The mice in the infection group were infected with P. berghei by injection through the tail vein (10 ⁶ parasites/mouse), while the control group received the same volume of saline. Six days after infection, spleens were collected to prepare single cell suspensions, and using flow cytometry to assay the amount of spleen B cells and NK cells and the expression of their surface molecules CD62L, CXCR3 and CD69. The results showed that the percentage of B cells in the infection group was (79.2 ± 3.6)%, significantly higher than that in the control group [(54.3 ± 4.4)%, P < 0.01]. The amount percentage of spleen NK cells in the infection group was (2.2 ± 0.7)%, significantly lower than that in the normal control group [(4.6 ± 0.8)%, P < 0.01]. The percentage of CD62L expression on B cells in the infection group was (77.7 ± 4.4)%, higher than that in the normal group (72.8 ± 7.1)%, but with no statistical significance (P > 0.05). The percentage of CD62L expression on NK cells in the infection group was (61.9 ± 4.8)%, which was significantly lower than that in the control group [(86.6 ± 4.2)%, P < 0.01]. The percentage of CXCR3 expression on B cells in the infection group was (4.1 ± 0.1)%, which was significantly higher than that in the control group [(3.0 ± 0.2) %, P < 0.01]. The percentage of CXCR3 expression on NK cells was (2.1 ± 0.9)%, which was lower than that of the control group [(2.3 ± 0.4)%], but with no statistical significance (P > 0.05). The percentages of CD69 expression on B cells and NK cells were (54.3 ± 4.7)% and (22.2 ± 1.6)%, respectively, both significantly higher than those in the control group [(1.9 ± 0.4)%, (1.3 ± 0.3)%, P < 0.01]. These results suggest that the mice infected with P. berghei may exert the affect against the infection through increasing B cell recruitment and up-regulating the expression of their surface molecules CXCR3 and CD69, and NK cell surface molecule CD69.

Key words: Plasmodium berghei, Spleen, Immune cells, Cell surface molecules

CLC Number:

R382.319

Jie-sen ZHANG, Meng-xin ZHANG, Meng-yi HAN, Bing-xia CHEN, Yong-sen LI, Chen-xi JIN, Yan-wei QI. Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(2): 255-258.

Figures/Tables 2

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