›› 2007, Vol. 25 ›› Issue (6): 17-512.

• 研究简报 • Previous Articles     Next Articles

A Modified Dye Test for Toxoplasma gondii Infection

XU Hui1 *,NI An-ping2,CUI Qing-tao2,HAO Ying2   

  1. 1 Department of Clinical Laboratory，Meitan General Hospital，Beijing 100028，China；2 Department of Clinical Laboratory，Peking Union Medical College Hospital，Chinese Academy of Medical Sciences，Beijing 100028，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-30 Published:2007-12-30

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Abstract: A modified dye test with microplate was to be established to detect Toxoplasma antibodies with cell-cultured Toxoplasma gondii. Numbers of stained and unstained tachyzoites were estimated in every 100 tachyzoites in each well after dyeing with methylene blue. The dilution with 50% tachyzoites stained was used as final dilution. Better results of the microplate dye test has been received when the concentration of tachyzoites in suspension reaches 109/ml with 1% sodium citrate as accessory factor.

Key words: Toxoplasma gondii, Dye test, Microplate