›› 2006, Vol. 24 ›› Issue (6): 12-456.

• 实验研究 • Previous Articles     Next Articles

Preparation and Identification of Monoclonal Antibody Against Sporozoites of Eimeria acervulina

WANG Cheng-min1 ;HE Hong-xuan2;QIN Jian-hua3;CHEN Gui-xiang1;
GUO Qi-zhen4;HANG Bo-lin1   

  1. 1 Cell Engineering Key Laboratory of Henan Institute of Science and Technology，Xinxiang 453003, China; 2 Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; 3 Animal Science and Technology School of Hebei Agricultural University，Baoding 071001, China; 4 Animal Husbandry Bureau of Xinxiang，Xinxiang 453003, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30
  • Contact: WANG Cheng-min

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Abstract: Objective To establish hybridoma cell lines against sporozoites of Eimeria acervulina. Methods BALB/c mice were immunized with purified sporozoites of E. acervulina. Hybridoma cell lines were set up by using hybridoma technique， and monoclonal antibodies were prepared. The monoclonal antibody was identified by determining their cross reactivity， relative affinity， immunoglobulin class or subclass with enzyme linked immunosorbent assay （ELISA）. Results Four hybridoma cell lines stably secreting McAbs against sporozoites were obtained: Easp-3G3 and Easp-5G10 belonging to IgG1， Easp-3H6 belonging to IgG2b， Easp-5H4 belonging to IgG2a. All four McAbs bound with E. acervulina sporozoite protein， but the Easp-5H4 McAb showed cross reactivity with E. tenellum sporozoite protein. Different antigenic epitopes were recognized by Easp-3G3 or Easp-5G10 and Easp-3H6 or Easp-5H4. Conclusion Three of the four produced monoclonal antibodies show high specificity and affinity to the Eimeria acervilina sporozoites.

Key words: Eimeria acervulina, Sporozoite, Monoclonal antibody, Identification