›› 2006, Vol. 24 ›› Issue (5): 18-390.

• 研究简报 • Previous Articles     Next Articles

Cloning and Sequence Analysis of a Partial Gene ofTrichomonas vaginalis dsRNA Virus

ZHAO Yue-ping1,2;ZHANG Xi-chen1;CHEN Li-feng1;LI Jian-hua1;YIN Ji-gang1;LIU Quan3;GONG Peng-tao1   

  1. 1 College of Animal Science and Veterinary Medicine，Jilin University，Changchun 130062，China；2 Hebei North University，Zhangjiakou 075000，China；3 Institute of Military Veterinary Medicine，Academy of Military Medical Sciences，Changchun 130062，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-30 Published:2006-10-30
  • Contact: ZHANG Xi-chen

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Abstract: A pair of degenerate primers were designed following the published nucleotide sequence of Trichomonas vaginalis virus（U08999， NC003873， NC003824， NC003834）. Using the total nucleic acids extracted from the Trichomonas vaginalis as template， RT-PCR was performed with the primers to obtain a fragment of the TVV. The product was cloned， sequenced and compared with the sequences available in the GenBank. The size of the amplified gene was 1 454bp， which shares 82.9% sequence identity with the Trichomonas vaginalis virus T1.

Key words: Trichomonas vaginalis, dsRNA virus, Cloning, Sequencing