Study on the Methods for Detection of Toxoplasma Antigen

›› 2006, Vol. 24 ›› Issue (1): 18-69.

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Study on the Methods for Detection of Toxoplasma Antigen

LI Hui;XU Bian-li;DENG Yan;ZHAO Xu-dong;LIN Xi-meng;YAN Xu-xia

Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450003,China

Received:1900-01-01 Revised:1900-01-01 Online:2006-02-28 Published:2006-02-28

Abstract

Abstract: A sandwich ELISA was established with monoclonal antibody as detecting antibody and rabbit anti- Toxoplasma polyclonal antibody as capturing antibody. ABC(avidin-biotin-peroxidase complex)-ELISA and immuno-PCR were also used to detect different concentrations of Toxoplasma antigen. The lowest concentration of antigen to be detected by sandwich ELISA, ABC-ELISA and immuno-PCR was 0.6 μg/ml, 0.075 μg/ml and 0.1 ng/ml respectively. The immuno-PCR shows a much higher sensitivity than the other two methods.

Key words: Toxoplasma, Antigen, Immunoassay

LIHui;XUBian-li;DENGYan;ZHAOXu-dong;LINXi-meng;YANXu-xia. Study on the Methods for Detection of Toxoplasma Antigen[J]. , 2006, 24(1): 18-69.

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Study on the Methods for Detection of Toxoplasma Antigen

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