Abstract: 　Objective To analyze the diversity of F1 in laboratory line Oncomelania hupensis hupensis . Methods Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes were amplified by polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed by GENETYX MAC software package (ver.9). Results Pairwise divergences among six F1 individuals were found in 12.2% nucleotides in COI gene fragments. 13.5% of genetic divergences between O.h.hupensis and O.h.robertsoni were identified. 94 amino acids were observed in difference. In Cyt b gene fragments, 6.4% of divergence was observed among four F1 samples. 25 amino acids were observed differently. The divergence of Cyt b genes from O.h.robertsoni and O.h.hupensis were 13.6%, included 6 amino acids. Conclusion Diversities were found in both COI and Cyt b gene sequences of F1 in laboratory line O.h.hupensis.

Key words: Snails, F1 filial generation, Genes, Genetic variation