Screening of cDNA Clone for Putative RNA Polymerase Subunit of Cysticercus cellulosae

Abstract

Abstract: 　Objective To obtain related genes of Cysticercus cellulosae from spliced leader (SL) cDNA library. Methods Spliced leader library of Cysticercus cellulosae was constructed using SL specific primer and oligo (dT)15 with M13M4 primer, and positive clones were then screened randomly, identified with enzyme restriction, followed by sequencing and homologous analysis. Results The amino acid sequence, encoded by the positive clone with a poly (A) 22 tail and a complete open reading frame (ORF), was with homology of RNA polymerase subunit genes of human, B. napus, fission yeast, A. thaliana, C. elegans and fruit fly up to 71.6%. Conclusion The protein, RNA polymerase subunit encoded putatively by the clone, is high conservative in different species.

Key words: Cysticercus cellulosae, RNA, Spliced Leader RNA, Gene Library, DNA-Directed RNA Polymerase

LUOXue-nong;ZHENGYa-dongDOUYong-xi;HOUJun-lin;JINGZhi-zhong.CAIXue-peng. Screening of cDNA Clone for Putative RNA Polymerase Subunit of Cysticercus cellulosae[J]. , 2004, 22(3): 9-163.

[1]	WU Aoxun, ZHONG Zifeng, XU Anyuan, PAN Xiaowen, YI Xueqing, WU Yinjuan, LI Xuerong. Research progress on the mechanism of Clonorchis sinensis miRNAs in the initiation and progression of cholangiocarcinoma [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 269-273.
[2]	ZHU Haotian, ZHOU Yongzhi, CAO Jie, WANG Ya’nan, ZHANG Houshuang, XU Qianming, ZHOU Jinlin. Identification of caspase 8 of Haemaphysalis longicornis and its role in resisting Babesia microti infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 433-438.
[3]	JIANG Nan, SU Yaxin, JIANG Xiaofeng, SHEN Yujuan, CAO Jianping. Heterogeneity analysis of T cells in liver of mice infected with Echinococcus granulosus based on single-cell RNA sequencing [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 286-294.
[4]	WU Yixuan, GUO Xiaola, CHEN Yixia. MicroRNA differential expression profiles and their diagnostic value in the sheep infected with Echinococcus granulosus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 295-302.
[5]	CHENG Donghui, JIANG Tiange, JING Yidan, YANG Limin, GUO Yunhai, FANG Yuan, LI Zhongqiu, ZHANG Yi. Transcriptome analysis of brain tissue in rat infected with Angiostrongylus cantonensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 217-224.
[6]	LIU Yi, CAI Yuchun, CHEN Jiaxu, CHEN Shaohong, YU Yingfang. Isolation, identification, and omics analysis of extracellular vesicles from Trichinella spiralis newborn larvae [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 225-233.
[7]	MIN Xiangyang, FENG Meng, ZHAO Xuhong, WENG Wenhao, LI Hanhua. Diagnosis of a case of Giardia lamblia infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 272-274.
[8]	WANG Yebin, SHEN Xuhang, DENG Guoqiang, HU Saimin, WANG Fei, ZHANG Lingling, SHEN Jilong. Effects of asymptomatic hookworm infection on intestinal microflora and metabolome in middle-aged and elderly people in rural area of Huangshan City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 17-26.
[9]	QIAO Tingting, TAO Xianglin, YE Changjiang, LI Zheng, ZHOU Xiaoyan, SUN Entao. Analysis on genetic variation and differentiation of Tyrophagus putrescentiae in different geographic populations [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 69-77.
[10]	WU Jiahui, SONG Xiao, CHENG Peng, LIU Hongmei, GUO Xiuxia, WANG Haifang, GONG Maoqing. Identification and analysis of miRNA targeting CYP450s genes in mosquitoes [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 683-690.
[11]	WANG Ning, PENG Hanqi, GAO Changzhe, CHENG Yuheng, LYU Dabing. Characterization of the mitochondrial genome and phylogenetic implication of Schistosoma japonicum featured with “nocturnal cercarial emergence” [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 699-707.
[12]	MA Zhiya, XIE Shichen, HE Yuanhui, GAO Wenwei, LIU Qing, ZHU Xingquan, ZHENG Wenbin. Infection status and genetic variation analysis of Haemonchus contortus in sheep in Shanxi Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 733-738.
[13]	YANG Shuo, XIA Shang, YAN Shuning, XUE Jingbo, SHI Benyun, HAO Yuwan, LI Mengru, LIANG Jiarui, XIA Zhigui, ZHENG Bin. Analysis on the sources of imported malaria risk in China based on international trade relations [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 744-748.
[14]	MA Yue, ZHAO Baocai, ZHOU Jiali, HU Junhao, ZHAO Hongxi. Research progress on the regulation of miRNA in the infection of apicomplexan parasites [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 749-755.
[15]	TAN Xiao, ZHU Qi, LIU Zhongqi, LI Jia, PENG Dingjin. Immunogenicity of Schistosoma japonicum Sj26gst mRNA vaccine candidate [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 546-551.

Screening of cDNA Clone for Putative RNA Polymerase Subunit of Cysticercus cellulosae

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments