›› 2002, Vol. 20 ›› Issue (5): 7-284.

• 论著 • Previous Articles     Next Articles

Construction of Cryptosporidium parvum cDNA Library and Cloning of P23、CP15/60

XU Wei dong 1;ZHANG Xi chen 1;KONG Qing chang 2;LIU Ming yuan 1;YIN Ji gang 1;LI Jian hua 1;YANG Ju 1;HE Hong xuan 1;L Ya jian 2   

  1. 1 Quatermaster University of PLA;Changchun 130062; 2 Veterinary Research Institute of Jilin Province;Changchun 130062
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-30 Published:2002-10-30

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Abstract: 　Objective To study the genetic background of Cryptosporidium parvum local strain and to provide a DNA source of the parasite. Methods With Promega′s kits, total RNAs and mRNAs were isolated from C. parvum and cDNAs were synthesized by reverse transcription. A cDNA library was prepared with pUC18 vector and host cells of E. coli strain DH5 alpha. Two PCR primers were designed and synthesized to screen protective genes. PCR products were cloned and sequenced. Results There are 1.9×10 6 recombinants in this library. The cDNA fragments ranged between 0.4×10 3 bp and 6.5×10 3 bp. Two genes encoding 23 kDa and 15/60 kDa sporozoite proteins of C. parvum respectively from the library were cloned and sequenced. Conclusion A cDNA library of Cryptosporidium parvum was prepared successfully.

Key words: Cryptosporidium parvum, cDNA library, sporozoite, surface antigen