Abstract: 　Objective] To screen and characterize immunodominant antigen epitopes on the soluble antigens of Trichinella spiralis (T s ) . [Methods]15 monoclonal antibodies (McAbs) against T s muscle larva(ML) soluble antigens were obtained by using hybridoma technique. The reactivity of monoclonal and polyclonal antibodies were tested by ELISA, Western blotting and indirect immunofluorescence assay(IFA). [Results] The Western blotting result showed that of the 15 McAbs, 6 could bind to the T s ML antigens displaying molecular weights of 40～70 kDa. Polyclonal sera could react with more than 10 bands having molecular weights of 20～200 kDa. Among the 6 McAbs, 4 could recognize epitopes on the cuticle surface and the other two could recognize epitopes on both the cuticle surface and the stichosome. [Conclusion]The antigen epitopes of T s recognized by 6 McAbs had been characterized.

Key words: Trichinella spiralis, epitope, monoclonal antibody, Western blotting, immunofluoscence