Prevalence of trematode metacercariae in Pseudorasbora parva and species identification in Qiqihaer area

doi:10.12140/j.issn.1000-7423.2023.01.018

Abstract

Abstract:

To understand the prevalence of trematode metacercariae and their species in Pseudorasbora parva from Nenjiang River around Qiqihaer City. P. parvas were collected from Nenjiang River around Qiqihaer City at the begining of each month from June to November in 2021. The prevalence of metacercaria of trematodes was examined by direct tablet, and the metacercaria of trematodes were isolated and purified by digestion. The metacercaria of trematodes were preliminarily classified by morphology. Mice (50-80 metacercaria/mouse) aged 6 weeks and ducks (80-100 metacercaria/duck) aged 2 weeks were fed with different types of metacercaria. The mice and ducks were dissected on the 30th and 25th day after infection, and the adult worms in the hepatobiliary duct were collected and stained with magenta acetate for morphological identification. The prevalence of metacercaria infection in P. parva was analyzed by month and season. SPSS 26.0 software was used for statistical analysis, and the prevalence was compared by χ² test. From June to November 2021, a total of 600 P. parva were collected, and 371 were infected with metacercaria, with an prevalence of 61.8%. A total of three different type metacercaria were obtained. According to the morphology, it was preliminarily identified as C. sinensis, M. orientalis, M. taiwanensis metacercaria, the prevalences were 47.7%(286/600), 15.5% (93/600) and 23.7% (142/600), respectively. The adult worms obtained from mice and ducks infected with the three types of metacercaria of trematodes were identified by morphology as C. sinensis, M. orientalis and M. taiwanensis. From June to November, the prevalences of C. sinensis metacercaria in P. parva were 26.0% (26/100), 31.0% (31/100), 52.0% (52/100), 66.0% (66/100), 63.0% (63/100) and 48.0% (48/100), respectively. The prevalences of M. orientalis metacercaria were 5.0% (5/100), 11.0% (11/100), 15.0% (15/100), 17.0% (17/100), 26.0% (26/100) and 19.0% (19/100), respectively. The prevalences of M. taiwanensis metacercaria were 17.0% (17/100), 15.0% (15/100) 19.0% (19/100), 23.0% (23/100), 32.0% (32/100) and 36.0% (36/100), the prevalences of the three metacercaria types in different months have statistical significance (χ² = 53.61, 19.51, 20.11; P < 0.05). The prevalences of metacercaria of C. sinensis, M. orientalis and M. taiwanensis in summer and autumn were 36.3% (109/300), 59.0% (177/300); 10.3% (31/300), 20.7% (62/300); 17.0% (51/300) and 30.3% (91/300), respectively, there were statistically significant differences in the prevalence of the three metacercaria in summer and autumn (χ² = 30.89, 12.23, 14.71; all P < 0.05). The difference of prevalences between three kinds of metacercaria in summer and autumn was statistically significant (χ² = 22.29, 33.96; all P < 0.05). P. parvas were infected with metacercaria of C. sinensis, M. orientalis, M. taiwanensis in the Qiqihar section of the Nenjiang River. The prevalence of three types of metacercaria were the highest in September, October and November respectively.

Key words: Pseudorasbora parva, Trematode, Metacercaria, Species, Infection

CLC Number:

R383.2

ZHANG Fengyu, LIU Liu, ZHANG Jing, ZHANG Hao. Prevalence of trematode metacercariae in Pseudorasbora parva and species identification in Qiqihaer area[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 112-116.

Figures/Tables 3

References 14

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特征	华支睾吸虫	东方次睾吸虫	台湾次睾吸虫
感染宿主	小鼠	雏鸭	雏鸭
虫体形态	背腹扁平，葵花籽状	叶状，前端较窄，后端钝圆	叶状，前端较窄，后端钝圆
虫体大小	（7~10）mm ×（1~3）mm	（4~7）mm ×（0.6~1.6）mm	（3~5）mm ×（0.5~1.4）mm
口吸盘	近圆形，大小（0.33 ± 0.07）mm ×（0.26 ± 0.55）mm	近圆形，大小（0.33 ± 0.06）mm × （0.31 ± 0.05）mm	杯状圆形，大小（0.21 ± 0.29）mm × （0.20 ± 0.03）mm
腹吸盘	近圆形，位于虫体前1/5，大小（0.26 ± 0.04）mm ×（0.24 ± 0.05）mm	近圆形，大小（0.28 ± 0.05）mm × （0.27 ± 0.04）mm	近圆形，大小（0.19 ± 0.03）mm × （0.18 ± 0.02）mm
睾丸	分枝，前睾丸分4枝，后睾丸分5~6枝	分瓣	圆形或椭圆形，边缘完整，个别略有分瓣
卵巢	边缘分叶，位于受精囊之前	圆形或椭圆形，大小约0.260 mm × 0.183 mm	圆形或椭圆形，大小约0.184 mm × 0.161 mm
子宫	从卵巢前段盘曲向前至腹吸盘处，不越过肠支	始于卵巢，越过肠管，达肠分支和腹吸盘之间，下降到腹吸盘前缘通生殖孔	始于卵巢水平，盘曲向前，两侧越过肠管，达肠分支与腹吸盘之间，至腹吸盘前方而后转折
卵黄腺	颗粒状滤泡组成，位于虫体两侧	椭圆形，分布于虫体中部2/4的两侧	椭圆形，分布于虫体两侧

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