CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2022, Vol. 40 ›› Issue (1): 43-49.doi: 10.12140/j.issn.1000-7423.2022.01.006
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SHAO Han1(), LI Si-yuan1, LI Jun2,*()
Received:
2021-06-18
Revised:
2021-07-25
Online:
2022-02-28
Published:
2022-01-13
Contact:
LI Jun
E-mail:1257460268@qq.com;xjlijun@163.com
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CLC Number:
SHAO Han, LI Si-yuan, LI Jun. The affect of metformin on autophagy and apoptosis of Echinococcus multilocularis cysts and protoscoleces[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 43-49.
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URL: https://www.jsczz.cn/EN/10.12140/j.issn.1000-7423.2022.01.006
Fig. 1
Affects of different metformin concentrations on the viability of Echinococcus multilocularis cysts(× 100) A, E: Control group; B-D: after 24 h of co-cultivation, the apoptotic cells in the cysts and the cysts turbidity increases as the concentration of metformin increases; F-H: after 48 h of co-cultivation, the cysts are less viable as metformin concentration increases and the formation of apoptotic bodies can be seen when metformin concentration reaches 10 mmol/L.
Fig. 2
Affect of different concentrations of metformin on the viability of protoscoleces of E. multilocularis (HE staining, × 100) A, E: Control group; B-D: After 24 h of co-cultivation, the proportion of red stained protoscoleces increases with the concentration of metformin; F-H: After co-cultivation with metformin for 48 h, the viability of protoscoleces reduced as the concentration of metformin increases, and all protoscoleces showed red-stained in the 10 mmol/L group.
Table 1
Expression of apoptotic proteins in E. multilocularis cysts and protoscoleces after treatment by metformin at different concentrations
蛋白Protein | 相对表达量 Relative expression | |||
---|---|---|---|---|
1 mmol/L二甲双胍 1 mmol/L Metformin | 5 mmol/L二甲双胍 5 mmol/L Metformin | 10 mmol/L二甲双胍 10 mmol/L Metformin | 对照组 Control group | |
囊泡 Vesicles B细胞淋巴瘤/白血病-2 Bcl-2 | 0.82 ± 0.05 | 0.89 ± 0.02 | 0.93 ± 0.04 | 1.09 ± 0.07 |
剪切的半胱氨酸肽酶3 Cleaved-caspase3 | 1.00 ± 0.09 | 1.34 ± 0.06 | 1.61 ± 0.05 | 0.67 ± 0.01 |
半胱氨酸肽酶9 Caspase9 | 0.94 ± 0.02 | 1.03 ± 0.11 | 0.78 ± 0.03 | 0.46 ± 0.05 |
原头节Protoscoleces B细胞淋巴瘤/白血病-2 Bcl-2 | 1.05 ± 0.12 | 0.60 ± 0.06 | 0.84 ± 0.12 | 1.55 ± 0.12 |
剪切的半胱氨酸肽酶3 Cleaved-caspase3 | 0.66 ± 0.09 | 0.80 ± 0.02 | 1.01 ± 0.05 | 0.46 ± 0.07 |
半胱氨酸肽酶9 Caspase9 | 0.72 ± 0.02 | 0.75 ± 0.05 | 0.97 ± 0.10 | 0.54 ± 0.07 |
Table 2
The expression of AMPK pathway-related proteins and autophagy-related proteins after E. multilocularis cysts and protoscoleces were treated with metformin at different concentrations
蛋白 Protein | 相对表达量 Relative expression | |||
---|---|---|---|---|
1 mmol/L二甲双胍 1 mmol/L Metformin | 5 mmol/L二甲双胍 5 mmol/L Metformin | 10 mmol/L二甲双胍 10 mmol/L Metformin | 对照组 Control group | |
囊泡 Vesicles 蛋白轻链3Ⅱ LC3-Ⅱ | 0.66 ± 0.06 | 0.86 ± 0.13 | 1.07 ± 0.06 | 0.75 ± 0.10 |
自噬基因14 Atg14 | 1.28 ± 0.21 | 1.33 ± 0.23 | 1.44 ± 0.23 | 0.52 ± 0.14 |
雷帕霉素机械靶蛋白 mTOR | 1.47 ± 0.27 | 1.42 ± 0.25 | 1.58 ± 0.31 | 0.63 ± 0.11 |
磷酸化的一磷酸腺苷依赖的蛋白激酶 P-AMPK | 0.77 ± 0.11 | 0.83 ± 0.12 | 0.69 ± 0.11 | 0.53 ± 0.09 |
原头节 Protoscoleces 蛋白轻链3Ⅱ LC3-Ⅱ | 0.84 ± 0.14 | 1.45 ± 0.23 | 1.68 ± 0.24 | 0.33 ± 0.07 |
自噬基因14 Atg14 | 0.89 ± 0.03 | 1.16 ± 0.10 | 1.33 ± 0.11 | 0.55 ± 0.09 |
雷帕霉素机械靶蛋白 mTOR | 1.10 ± 0.02 | 1.49 ± 0.11 | 1.25 ± 0.03 | 0.59 ± 0.06 |
磷酸化的一磷酸腺苷依赖的蛋白激酶 P-AMPK | 1.18 ± 0.09 | 1.52 ± 0.09 | 1.27 ± 0.19 | 0.20 ± 0.01 |
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