河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

doi:10.12140/j.issn.1000-7423.2023.05.007

中国寄生虫学与寄生虫病杂志 ›› 2023, Vol. 41 ›› Issue (5): 567-572.doi: 10.12140/j.issn.1000-7423.2023.05.007

河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

纪鹏慧(), 蒋甜甜, 贺志权, 王丹, 岳思宁, 李素华, 杨成运, 王昊, 张红卫, 周瑞敏^*()

河南省疾病预防控制中心，河南省寄生虫病原与媒介医学重点实验室，河南省传染病病原生物重点实验室，郑州 450016

收稿日期:2023-04-03 修回日期:2023-09-09 出版日期:2023-10-30 发布日期:2023-11-06
通讯作者: *周瑞敏（1983-），女，硕士，副主任医师，从事寄生虫病防治和研究工作。E-mail：zrm920@126.com
作者简介:纪鹏慧（1991-），女，硕士，医师，从事寄生虫病防治和研究工作。E-mail：614936320@qq.com
基金资助:
河南省医学科技攻关计划联合共建项目(LHGJ20210134);河南省医学科技攻关计划联合共建项目(LHGJ20220157)

Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province

JI Penghui(), JIANG Tiantian, HE Zhiquan, WANG Dan, YUE Sining, LI Suhua, YANG Chengyun, WANG Hao, ZHANG Hongwei, ZHOU Ruimin^*()

Henan Center for Disease Control and Prevention, Henan Provincial Medical Key Laboratory of Parasitic Pathogen and Vector Provincial Key Laboratory for Infectious Disease Prevention and Control, Zhengzhou 450016, China

Received:2023-04-03 Revised:2023-09-09 Online:2023-10-30 Published:2023-11-06
Contact: *E-mail: zrm920@126.com
Supported by:
Henan Province Medical Science and Technology Research Plan Joint Construction Project(LHGJ20210134);Henan Province Medical Science and Technology Research Plan Joint Construction Project(LHGJ20220157)

摘要/Abstract

摘要：

目的了解河南省信阳地区家畜寄生蜱感染巴贝虫的情况。方法 2022年6—8月在河南省信阳市光山县和商城县采集家畜动物体表寄生蜱，采用形态学和PCR扩增蜱虫16S rDNA鉴定蜱虫种类。提取蜱虫基因组DNA，采用巢式PCR扩增巴贝虫18S rRNA基因。目的条带经测序后，进行BLAST比对分析，采用邻接法构建系统进化树，采用MEGA7.0软件进行同源性分析。结果共釆集335只蜱，形态学和PCR扩增鉴定结果显示，长角血蜱49只、微小扇头蜱208只、褐黄血蜱1只、具环扇头蜱34只、刻点血蜱43只。PCR扩增结果显示，335份蜱虫样品中有2份样品扩增出巴贝虫400 bp大小的目的条带，蜱虫巴贝虫总阳性率为0.6%。BLAST比对分析结果显示，1份样品扩增出的18S rRNA序列与GenBank中来自美国（MK609547）、泰国（MG199181）、中国（KU204794）的田鼠巴贝虫序列相似性均达99.26%；另1份样品扩增出的18S rRNA序列与GenBank中来自美国（MH620203）、印度（MN161136）和中国（KP666166）的吉氏巴贝虫序列相似性均达100%。系统进化树分析结果显示，田鼠巴贝虫阳性样品与来自俄罗斯（KX987864）、中国（KU204794）、泰国（MG199179）等地的田鼠巴贝虫聚在同一分支上，同源性较高；吉氏巴贝虫阳性样品与来自中国（FJ769386）、美国（MH620203）和印度（KF606884）等地的吉氏巴贝虫聚在同一分支上，同源性较高。结论河南省信阳地区家畜寄生蜱存在田鼠巴贝虫和吉氏巴贝虫感染，可能对人和家畜有一定的致病风险。

关键词: 蜱, 巴贝虫, 家畜, 分子流行病学, 信阳, 河南省

Abstract:

Objective To understand the infection of Babesia in ticks parasitized on domestic animals in Xinyang, Henan Province. Methods Tick specimens were collected from domestic animals in Guangshan County and Shangcheng County, Xinyang City, Henan Province, from June to August 2022. The tick species were identified by morphology and 16S rDNA. Genomic DNA was extracted from tick specimens, and nested-PCR was performed to amplify 18S rRNA gene sequence of Babesia. The PCR products were sequenced and aligned by BLAST, and phylogenetic trees were constructed by the neighbor-joining method. Using MEGA7.0 software for homology analysis. Results A total of 335 ticks were collected, including the species of 49 Haemaphysalis longicornis, 208 Rhipicephalus microplus, 1 H. flava, 34 R. annulatus, and 43 H. punctataa, identified by morphology and PCR amplification. The PCR amplification results showed that 2 out of 335 tick samples were amplified target bands of 400 bp, and the overall positive rate was 0.6%. The BLAST comparison analysis results showed that the 18S rRNA sequence amplified from one sample had a 99.26% similarity with the sequences of B. microti from the United States (MK609547), Thailand (MG199181), and China (KU204794) in GenBank. The 18S rRNA sequence amplified from another sample shares 100% similarity with the sequences of B. gibsoni from the United States (MH620203), India (MN161136), and China (KP666166) in GenBank. The phylogenetic tree analysis revealed that the B. microti positive sample was clustered on the same branch with B. microti from Russia (KX987864), China (KU204794) and Thailand (MG199179), showing a high homology. The B. gibsoni-positive samples were clustered on the same branch with B. gibsoni from China (FJ769386), USA (MH620203) and India (KF606884), showing a close relation. Conclusion The infections of B. microti and B. gibsoni were detected in ticks parasitized on domestic animals in Xinyang area of Henan Province, which may render risk of disease transmission to humans and domestic animals.

Key words: Tick, Babesia, Domestic animals, Molecular epidemiology, Xinyang, Henan Province

中图分类号:

R531.5

纪鹏慧, 蒋甜甜, 贺志权, 王丹, 岳思宁, 李素华, 杨成运, 王昊, 张红卫, 周瑞敏. 河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 567-572.

JI Penghui, JIANG Tiantian, HE Zhiquan, WANG Dan, YUE Sining, LI Suhua, YANG Chengyun, WANG Hao, ZHANG Hongwei, ZHOU Ruimin. Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2023, 41(5): 567-572.

图/表 3

参考文献 37

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采样点 Site of collection	宿主 Host	蜱虫数量/只 No. tick
采样点 Site of collection	宿主 Host	长角血蜱 H. longicornis	微小扇头蜱 R. microplus	具环扇头蜱 R. annulatus	褐黄血蜱 H. flava	刻点血蜱 H. punctata	合计 Total
光山县横大路村 Hengdalu Village, Guangshan County	羊 Goat	8	0	0	0	8	16
光山县大刘村 Daliu Village, Guangshan County	羊、犬 Goat、Dog	20	0	0	1	30	51
商城县徐堰村 Xuyan Village, Shangcheng County	羊 Goat	18	1	0	0	5	24
商城县石关口村 Shiguankou Village, Shangcheng County	黄牛 Yellow cattle	3	207	34	0	0	244
合计 Total		49	208	34	1	43	335

河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province

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