河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

doi:10.12140/j.issn.1000-7423.2023.05.007

中国寄生虫学与寄生虫病杂志 ›› 2023, Vol. 41 ›› Issue (5): 567-572.doi: 10.12140/j.issn.1000-7423.2023.05.007

河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

纪鹏慧(), 蒋甜甜, 贺志权, 王丹, 岳思宁, 李素华, 杨成运, 王昊, 张红卫, 周瑞敏^*()

河南省疾病预防控制中心，河南省寄生虫病原与媒介医学重点实验室，河南省传染病病原生物重点实验室，郑州 450016

收稿日期:2023-04-03 修回日期:2023-09-09 出版日期:2023-10-30 发布日期:2023-11-06
通讯作者: *周瑞敏（1983-），女，硕士，副主任医师，从事寄生虫病防治和研究工作。E-mail：zrm920@126.com
作者简介:纪鹏慧（1991-），女，硕士，医师，从事寄生虫病防治和研究工作。E-mail：614936320@qq.com
基金资助:
河南省医学科技攻关计划联合共建项目(LHGJ20210134);河南省医学科技攻关计划联合共建项目(LHGJ20220157)

Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province

JI Penghui(), JIANG Tiantian, HE Zhiquan, WANG Dan, YUE Sining, LI Suhua, YANG Chengyun, WANG Hao, ZHANG Hongwei, ZHOU Ruimin^*()

Henan Center for Disease Control and Prevention, Henan Provincial Medical Key Laboratory of Parasitic Pathogen and Vector Provincial Key Laboratory for Infectious Disease Prevention and Control, Zhengzhou 450016, China

Received:2023-04-03 Revised:2023-09-09 Online:2023-10-30 Published:2023-11-06
Contact: *E-mail: zrm920@126.com
Supported by:
Henan Province Medical Science and Technology Research Plan Joint Construction Project(LHGJ20210134);Henan Province Medical Science and Technology Research Plan Joint Construction Project(LHGJ20220157)

摘要/Abstract

摘要：

目的了解河南省信阳地区家畜寄生蜱感染巴贝虫的情况。方法 2022年6—8月在河南省信阳市光山县和商城县采集家畜动物体表寄生蜱，采用形态学和PCR扩增蜱虫16S rDNA鉴定蜱虫种类。提取蜱虫基因组DNA，采用巢式PCR扩增巴贝虫18S rRNA基因。目的条带经测序后，进行BLAST比对分析，采用邻接法构建系统进化树，采用MEGA7.0软件进行同源性分析。结果共釆集335只蜱，形态学和PCR扩增鉴定结果显示，长角血蜱49只、微小扇头蜱208只、褐黄血蜱1只、具环扇头蜱34只、刻点血蜱43只。PCR扩增结果显示，335份蜱虫样品中有2份样品扩增出巴贝虫400 bp大小的目的条带，蜱虫巴贝虫总阳性率为0.6%。BLAST比对分析结果显示，1份样品扩增出的18S rRNA序列与GenBank中来自美国（MK609547）、泰国（MG199181）、中国（KU204794）的田鼠巴贝虫序列相似性均达99.26%；另1份样品扩增出的18S rRNA序列与GenBank中来自美国（MH620203）、印度（MN161136）和中国（KP666166）的吉氏巴贝虫序列相似性均达100%。系统进化树分析结果显示，田鼠巴贝虫阳性样品与来自俄罗斯（KX987864）、中国（KU204794）、泰国（MG199179）等地的田鼠巴贝虫聚在同一分支上，同源性较高；吉氏巴贝虫阳性样品与来自中国（FJ769386）、美国（MH620203）和印度（KF606884）等地的吉氏巴贝虫聚在同一分支上，同源性较高。结论河南省信阳地区家畜寄生蜱存在田鼠巴贝虫和吉氏巴贝虫感染，可能对人和家畜有一定的致病风险。

关键词: 蜱, 巴贝虫, 家畜, 分子流行病学, 信阳, 河南省

Abstract:

Objective To understand the infection of Babesia in ticks parasitized on domestic animals in Xinyang, Henan Province. Methods Tick specimens were collected from domestic animals in Guangshan County and Shangcheng County, Xinyang City, Henan Province, from June to August 2022. The tick species were identified by morphology and 16S rDNA. Genomic DNA was extracted from tick specimens, and nested-PCR was performed to amplify 18S rRNA gene sequence of Babesia. The PCR products were sequenced and aligned by BLAST, and phylogenetic trees were constructed by the neighbor-joining method. Using MEGA7.0 software for homology analysis. Results A total of 335 ticks were collected, including the species of 49 Haemaphysalis longicornis, 208 Rhipicephalus microplus, 1 H. flava, 34 R. annulatus, and 43 H. punctataa, identified by morphology and PCR amplification. The PCR amplification results showed that 2 out of 335 tick samples were amplified target bands of 400 bp, and the overall positive rate was 0.6%. The BLAST comparison analysis results showed that the 18S rRNA sequence amplified from one sample had a 99.26% similarity with the sequences of B. microti from the United States (MK609547), Thailand (MG199181), and China (KU204794) in GenBank. The 18S rRNA sequence amplified from another sample shares 100% similarity with the sequences of B. gibsoni from the United States (MH620203), India (MN161136), and China (KP666166) in GenBank. The phylogenetic tree analysis revealed that the B. microti positive sample was clustered on the same branch with B. microti from Russia (KX987864), China (KU204794) and Thailand (MG199179), showing a high homology. The B. gibsoni-positive samples were clustered on the same branch with B. gibsoni from China (FJ769386), USA (MH620203) and India (KF606884), showing a close relation. Conclusion The infections of B. microti and B. gibsoni were detected in ticks parasitized on domestic animals in Xinyang area of Henan Province, which may render risk of disease transmission to humans and domestic animals.

Key words: Tick, Babesia, Domestic animals, Molecular epidemiology, Xinyang, Henan Province

中图分类号:

R531.5

纪鹏慧, 蒋甜甜, 贺志权, 王丹, 岳思宁, 李素华, 杨成运, 王昊, 张红卫, 周瑞敏. 河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 567-572.

JI Penghui, JIANG Tiantian, HE Zhiquan, WANG Dan, YUE Sining, LI Suhua, YANG Chengyun, WANG Hao, ZHANG Hongwei, ZHOU Ruimin. Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2023, 41(5): 567-572.

图/表 3

参考文献 37

[1]	Barreira JD, Rossi MI, Pires FA, et al. The effect of different strains Babesia bovis (Babes, 1888) on tick of Boophilus microplus (Canestrini, 1887)[J]. Rev Bras Parasitol Vet, 2006, 15(4): 138-142.
[2]	Bläckberg J, Lazarevic VL, Hunfeld KP, et al. Low-virulent Babesia venatorum infection masquerading as hemophagocytic syndrome[J]. Ann Hematol, 2018, 97(4): 731-733. doi: 10.1007/s00277-017-3220-6 pmid: 29285582
[3]	Bloch EM, Kasubi M, Levin A, et al. Babesia microti and malaria infection in Africa: a pilot serosurvey in Kilosa District, Tanzania[J]. Am J Trop Med Hyg, 2018, 99(1): 51-56. doi: 10.4269/ajtmh.18-0012
[4]	O'Connell S, Lyons C, Abdou M, et al. Splenic dysfunction from celiac disease resulting in severe babesiosis[J]. Ticks Tick Borne Dis, 2017, 8(4): 537-539. doi: 10.1016/j.ttbdis.2017.02.016
[5]	Morters MK, Archer J, Ma D, et al. Long-term follow-up of owned, free-roaming dogs in South Africa naturally exposed to Babesia rossi[J]. Int J Parasitol, 2020, 50(2): 103-110. doi: S0020-7519(20)30008-4 pmid: 32004510
[6]	Skrabalo Z, Deanovic Z. Piroplasmosis in man: report of a case[J]. Doc Med Geogr Trop, 1957, 9(1): 11-16.
[7]	Lobo CA, Singh M, Rodriguez M. Human babesiosis: recent advances and future challenges[J]. Curr Opin Hematol, 2020, 27(6): 399-405. doi: 10.1097/MOH.0000000000000606 pmid: 32889826
[8]	Santos MA, Tierney LM, Manesh R. Babesiosis-associated warm autoimmune hemolytic Anemia[J]. J Gen Intern Med, 2020, 35(3): 928-929. doi: 10.1007/s11606-019-05506-5
[9]	Häselbarth K, Tenter AM, Brade V, et al. First case of human babesiosis in Germany: clinical presentation and molecular characterisation of the pathogen[J]. Int J Med Microbiol, 2007, 297(3): 197-204. pmid: 17350888
[10]	Kukina IV, Guzeeva TM, Zelya OP, et al. Fatal human babesiosis caused by Babesia divergens in an asplenic host[J]. IDCases, 2018, 13: e00414. doi: 10.1016/j.idcr.2018.e00414
[11]	Bondarenko AV, Torianyk II, Pokhil SI, et al. Seroprevalence of babesiosis in immunocompetent and immunocompromised individuals[J]. Pol Merkur Lekarski, 2021, 49(291): 193-197. pmid: 34218237
[12]	Young KM, Corrin T, Wilhelm B, et al. Zoonotic Babesia: a scoping review of the global evidence[J]. PLoS One, 2019, 14(12): e0226781. doi: 10.1371/journal.pone.0226781
[13]	Smith RP, Hunfeld KP, Krause PJ. Management strategies for human babesiosis[J]. Expert Rev Anti Infect Ther, 2020, 18(7): 625-636. doi: 10.1080/14787210.2020.1752193 pmid: 32268823
[14]	Madison-Antenucci S, Kramer LD, Gebhardt LL, et al. Emerging tick-borne diseases[J]. Clin Microbiol Rev, 2020, 33(2): e00083.
[15]	Zheng WQ, Umemiya-Shirafuji R, Zhang Q, et al. Porin expression profiles in Haemaphysalis longicornis infected with Babesia microti[J]. Front Physiol, 2020, 11: 502. doi: 10.3389/fphys.2020.00502
[16]	Xu BL, Liu LC, Huang XY, et al. Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus[J]. PLoS Pathog, 2011, 7(11): e1002369. doi: 10.1371/journal.ppat.1002369
[17]	Li SH, Zhao YL, Gao LJ, et al. Analysis of molecular epidemiology of babesiosis in patients having fever and thrombocytopenia in Xinyang City, Henan Province[J]. Chin J Parasitol Parasit Dis, 2019, 37(1): 66-69. (in Chinese)
	(李素华, 赵玉玲, 高丽君, 等. 河南省信阳市发热伴血小板减少患者中巴贝虫感染的分子流行特征分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(1): 66-69.)
[18]	Deng GP, Jiang ZJ. Economic insect fauna of China, fasc 39,Acari: Ixodidae[M]. Beijing: Science Press, Academia Sinica, 1991: 1-359. (in Chinese)
	(邓国藩, 姜在阶. 中国经济昆虫志, 第39册, 蜱螨亚纲, 硬蜱科[M]. 北京: 科学出版社, 1991: 1-359.)
[19]	Liu Q, Zhang Y, Fang Y, et al. Identification of Haemaphysalis longicornis, H. flava and H. campanulata based on molecular markers of 16S rDNA and COⅠ gene[J]. Int J Med Parasit Dis, 2015, 42(3): 146-151. (in Chinese)
	(刘琴, 张仪, 方圆, 等. 基于16S rDNA和COⅠ基因的3种血蜱分子生物学鉴定[J]. 国际医学寄生虫病杂志, 2015, 42(3): 146-151.)
[20]	Zhang CC, Zhang Y. Research progress on biological chracteristics of common tick species and tick-borne diseases in China[J]. Int J Med Parasit Dis, 2015, 42(3): 184-188. (in Chinese)
	(张臣臣, 张仪. 我国常见蜱的生物学特性及其传播疾病研究进展[J]. 国际医学寄生虫病杂志, 2015, 42(3): 184-188.)
[21]	Zhao Q, Gao LJ, Tang ZQ, et al. Investigation of species, temporal and spatial distribution of ticks in Henan Province, China[J]. Chin J Vector Biol Contr, 2015, 26(1): 75-77. (in Chinese)
	(赵奇, 高丽君, 唐振强, 等. 河南省蜱种类和地理分布及季节消长调查[J]. 中国媒介生物学及控制杂志, 2015, 26(1): 75-77.) doi: 10.11853/j.issn.1003.4692.2015.01.020
[22]	Gao LJ, Zhao Q, Li SH, et al. Investigation on the selectivity of Haemaphysalis longicornis for hosts in different geographic zoning of Henan[J]. Henan J Prev Med, 2018, 29(9): 671-672, 730. (in Chinese)
	(高丽君, 赵奇, 李素华, 等. 河南省不同地理区划长角血蜱对宿主选择性的研究[J]. 河南预防医学杂志, 2018, 29(9): 671-672, 730.)
[23]	Lu M, Tian JH, Pan XL, et al. Identification of Rickettsia spp., Anaplasma spp., and an Ehrlichia canis-like agent in Rhipicephalus microplus from southwest and south-central China[J]. Ticks Tick Borne Dis, 2022, 13(2): 101884. doi: 10.1016/j.ttbdis.2021.101884
[24]	Chen Z. Investigation of piroplasms in different hosts in Xinyang, Henan Province[D]. Beijing: Chinese Center for Disease Control and Prevention, 2014: 38-44. (in Chinese)
	(陈卓. 河南省信阳地区不同宿主梨形虫感染研究[D]. 北京: 中国疾病预防控制中心, 2014: 38-44.)
[25]	Fu TT, Bai XG, Tang F, et al. Investigation on vector tick and tick-borne pathogens in sheep in some regions of Shaanxi and Liaoning Province[J]. Chin J Vet Med, 2023, 59(6): 1-8. (in Chinese)
	(付婷婷, 白新鸽, 汤芳, 等. 陕西和辽宁部分地区羊源媒介蜱类及其携带病原体调查[J]. 中国兽医杂志, 2023, 59(6): 1-8.)
[26]	Zhang JW, Liu QB, Wang DM, et al. Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China[J]. Parasite, 2017, 24: 35. doi: 10.1051/parasite/2017036 pmid: 28971797
[27]	Groves MG, Yap LF. Babesia gibsoni (Patton, 1910) from a dog in Kuala Lumpur[J]. Med J Malaya, 1968, 22(3): 229. pmid: 4234362
[28]	Kjemtrup AM, Kocan AA, Whitworth L, et al. There are at least three genetically distinct small piroplasms from dogs[J]. Int J Parasitol, 2000, 30: 1501-1505. doi: 10.1016/s0020-7519(00)00120-x pmid: 11428342
[29]	Muhlnickel CJ, Jefferies R, Morgan-Ryan UM, et al. Babesia gibsoni infection in three dogs in Victoria[J]. Aust Vet J, 2002, 80(10): 606-610. pmid: 12465811
[30]	Xu D, Zhang JL, Shi ZS, et al. Molecular detection of vector-borne agents in dogs from ten provinces of China[J]. Parasit Vectors, 2015, 8: 501. doi: 10.1186/s13071-015-1120-y pmid: 26428085
[31]	He L, Miao XY, Hu JF, et al. First molecular detection of Babesia gibsoni in dogs from Wuhan, China[J]. Front Microbiol, 2017, 8: 1577. doi: 10.3389/fmicb.2017.01577
[32]	Li J, Kelly P, Zhang JL, et al. Development of a pan-Babesia FRET-qPCR and a survey of livestock from five Caribbean Islands[J]. BMC Vet Res, 2015, 11: 246. doi: 10.1186/s12917-015-0560-0
[33]	Solano-Gallego L, Baneth G. Babesiosis in dogs and cats: expanding parasitological and clinical spectra[J]. Vet Parasitol, 2011, 181(1): 48-60. doi: 10.1016/j.vetpar.2011.04.023 pmid: 21571435
[34]	Zhou X, Wang H, Xue JB, et al. Epidemic and research progress of babesiosis[J]. Chin J Schisto Control, 2019, 31(1): 63-70. (in Chinese)
	(周霞, 王慧, 薛靖波, 等. 国内外巴贝虫病流行现状与研究进展[J]. 中国血吸虫病防治杂志, 2019, 31(1): 63-70.)
[35]	Huang SS, Zhang LL, Yao LN, et al. Human babesiosis in Southeast China: a case report[J]. Int J Infect Dis, 2018, 68: 36-38. doi: S1201-9712(18)30004-3 pmid: 29355731
[36]	Ouyang R, Chen ZY, Lin YY, et al. Diagnosis and identification for human babesiosis in Fujian Province, China[J]. Chin J Zoonoses, 2018, 34(5): 492-494. (in Chinese)
	(欧阳榕, 陈朱云, 林耀莹, 等. 福建省1例人巴贝虫病的诊断与鉴定[J]. 中国人兽共患病学报, 2018, 34(5): 492-494.)
[37]	Jia N, Zheng YC, Jiang JF, et al. Human babesiosis caused by a Babesia crassa-like pathogen: a case series[J]. Clin Infect Dis, 2018, 67(7): 1110-1119. doi: 10.1093/cid/ciy212 pmid: 29538646

采样点 Site of collection	宿主 Host	蜱虫数量/只 No. tick
采样点 Site of collection	宿主 Host	长角血蜱 H. longicornis	微小扇头蜱 R. microplus	具环扇头蜱 R. annulatus	褐黄血蜱 H. flava	刻点血蜱 H. punctata	合计 Total
光山县横大路村 Hengdalu Village, Guangshan County	羊 Goat	8	0	0	0	8	16
光山县大刘村 Daliu Village, Guangshan County	羊、犬 Goat、Dog	20	0	0	1	30	51
商城县徐堰村 Xuyan Village, Shangcheng County	羊 Goat	18	1	0	0	5	24
商城县石关口村 Shiguankou Village, Shangcheng County	黄牛 Yellow cattle	3	207	34	0	0	244
合计 Total		49	208	34	1	43	335

河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查

Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang, Henan Province

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 37

相关文章 15

编辑推荐

Metrics

本文评价

[1]	费思伟, 赵翰卿, 尹静娴, 孙芷珊, 郭晓奎, KASSEGNE Kokouvi, 周晓农. 基于文献计量分析的蜱及蜱传疾病研究领域发展趋势[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 609-618.
[2]	刘岳青, 马林源, 陈开廷, 高金亮, 王鹏. 蜱源Kunitz型丝氨酸蛋白酶抑制分子的结构与功能研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 625-630.
[3]	徐以勒, 李江峰, 张家祺, 余向华, 阮卫. 晚期癌症患者合并巴贝虫感染1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 393-396.
[4]	孙家辉, 宋鹏, 陈木新, 周魇, 林琳, 陈家旭, 蔡玉春. 田鼠巴贝虫肽基脯氨酸异构酶基因的重组表达及功能分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 29-35.
[5]	蒋甜甜, 纪鹏慧, 贺志权, 陈伟奇, 张雅兰, 邓艳, 王丹, 周瑞敏, 刘颖, 张红卫. 2016—2020年河南省土源性线虫感染监测结果分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 742-747.
[6]	纪鹏慧, 蒋甜甜, 贺志权, 周瑞敏, 李素华, 杨成运, 钱丹, 刘颖, 王昊, 张红卫. 2011—2021年河南省输入性三日疟流行病学特征分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 801-805.
[7]	刘雅芳, 陈彬, 芦新焱, 李光华, 杜春红, 姜丹丹, 杨兴. 云南微小扇头蜱线粒体基因组全序列测定与分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 677-681.
[8]	杨成运, 贺志权, 鲁德领, 钱丹, 刘颖, 李素华, 周瑞敏, 邓艳, 张红卫, 王昊, 赵东阳, 郭万申. 2020年河南省内脏利什曼病病例的流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 481-486.
[9]	宋鹏, 蔡玉春, 卢艳, 艾琳, 陈木新, 陈韶红, 陈家旭. 田鼠巴贝虫丽水分离株小鼠感染模型的建立及其病理变化[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 493-499.
[10]	宋瑞其, 翟雪洁, 李才善, 葛婷, 甘露, 张梦圆, 樊新丽, 李永畅, 张杨, 巴音查汗. 亚洲璃眼蜱和小亚璃眼蜱新疆地理株各发育阶段生物学特性的比较分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 369-378.
[11]	旷策嫣, 周金林. 蜱的化学感觉系统与驱避剂的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 104-108.
[12]	何文文, 伍军, 呼尔查, 阿力木江, 史倩云, 诺明达来, 甘露, 郝蕴伟, 巴音查汗. 基于最大熵模型的新疆银盾革蜱和边缘革蜱生境适应性评价[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 68-75.
[13]	周淑姮, 曾志伟, 刘维俊, 王加熊, 徐国英, 肖方震. 闽东地区野生动物寄生蜱感染梨形虫状况的调查及基因型分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 76-83.
[14]	殷梦, 张皓冰, 陶奕, 姜斌, 刘华. 马拉龙和阿托伐醌 + 阿奇霉素在不同免疫状态小鼠体内的抗田鼠巴贝虫药效评价[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(5): 659-665.
[15]	吕立红, 张金成, 胡永红. 蜱类保护性抗原研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(4): 542-547.