关键词: 恶性疟原虫, 滤纸干血滴, 蛋白酶, 抽提方法, 凝胶电泳, 金属离子, 琼脂糖, 弱阳离子交换剂, 采血方法, 特异性

Abstract: Polymerase chain reaction (PCR) primered with primers 3,4 and 5,6 was performed using DNA extracted from dried blood spot of a falciparum malaria patient from Mengpeng Township,Yunnan using four different methods,including STE proteinase K SDS,methanol proteinase K SDS,Chelex 100 and Chelex 100 proteinase K to amplify DNA of apical membrane antigen 1(AMA 1).A 900 bp DNA band could be seen on the ethidium bromide stained agarose gel electrophoresis of the PCR products when DNA was extracted using all the above mentioned methods except STE proteinase K SDS method. DNA extracted with Chelax 100 was recommended.