CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2022, Vol. 40 ›› Issue (5): 587-593.doi: 10.12140/j.issn.1000-7423.2022.05.004
• ORIGINAL ARTICLES • Previous Articles Next Articles
ZOU Wei-hao(), WU Wei-ling, LIAO Yuan-peng, CHEN Min, PENG Hong-juan()
Received:
2021-12-20
Revised:
2022-01-21
Online:
2022-10-30
Published:
2022-10-17
Contact:
PENG Hong-juan
E-mail:544273235@qq.com;hongjuan@smu.edu.cn
Supported by:
CLC Number:
ZOU Wei-hao, WU Wei-ling, LIAO Yuan-peng, CHEN Min, PENG Hong-juan. Preparation and application of monoclonal antibody against Toxoplasma gondii bradyzoite antigen 1[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 587-593.
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URL: https://www.jsczz.cn/EN/10.12140/j.issn.1000-7423.2022.05.004
Fig. 2
Detection of Toxoplasma gondii BAG1 expression by SDS-PAGE M: Protein markers; 1, 2: The total protein of ultrasonic cell lysate of pET28a(+) blank vector without and with induction; 3, 4: The total ultrasonic cell lysate of pET28a(+)-bag1 recombinant E. coli without and with induction; 5, 6: The ultrasonic cell lysissupernatant of pET28a(+)-bag1 recombinant E. coli without and with induction; 7, 8: The ultrasonic cell lysisprecipitation of pET28a(+)-bag1 recombinant E. coli without and with induction.
Fig. 3
Detection of the purified Toxoplasma gondii BAG1 protein by SDS-PAGE M: Protein markers; 1: Supernatant of ultrasonic lysed recombinant E. coli after IPTG induction; 2: Flow through collected from the Ni-NTA column; 3: Washing samples with 50 mmol/L imidazole buffer; 4-8: Elution samples with 250 mmol/L imidazole elution.
Fig. 7
Immuno-fluorescence assay showing mAb4B04 recognize ME49 bradyzoites induced by alkaline medium (× 1 000)Note: The primary antibody used for the red fluorescent channel was mAb4B04 monoclonal antibody, and the primary antibody used for the green fluorescent channel was anti-Toxoplasma gondii tachyzoite antigen polyclonal antibody. Tachyzoite; Bradyzoites.
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