Investigation and genetic identification on Babesia infection in rodents in some areas of Fujian Province

Abstract

Abstract:

Objective To explore the status of Babesia infection in rodents and the genetic characteristics of Babesia spp. in Fujian Province. Methods Rodents were captured by the night trapping method in Shaowu, Qingliu, Shunchang, Yong’an, Changle and Youxi during 2014-2015. The rodent species was identified, and information on the time and place of capture, species and sex of rodents was recorded. Blood samples was collected, in which the fragment of 18S rRNA gene of Babesia spp. was amplified by PCR. The PCR products were sequenced and the phylogenetic tree was constructed for homology analysis. Data on positive rate were analyzed with Chi-square or Fisher exact test. Results Two hundred and nine rats were captured, comprising of 71 domestic and 138 wild rats. The overall positive rate was 9.6% （20/209）. The positive rate in domestic rats was 2.8% （2/71）, including one Rattus norvebicus and one Rattus flavipectus. The positive rate in wild rats was 13.0% （18/138）, including 13 Bandicota indica, one Rattus losea, 2 Rattus confucianus and 2 Rattus fulvescens. The positive rate was significantly higher in wild rats than in domestic rats （P < 0.05）. The Youxi region had the highest positive rate（14.9%, 13/87）, followed by Yong’an（13.6%, 3/22）, and no positive rat was found in Qingliu. The positive rate in the male rats was 7.9% （9/114）, and that in the females was 11.6% （11/95）. The positive rate was highest in adult rats （10.4%, 18/173）, followed by young ones （6.3%, 2/32）. No positive rat was found in old rats. There was no significant difference in positive rate among different regions, between male and female rats, or among different ages （P > 0.05）. The sequences of PCR products had a 100% homology. The BLAST results revealed the species to be Babesia microti. The phylogenetic tree showed that the sample sequence was the most homologous with Babesia microti from Zhejiang Province（GenBank Accession No: JQ609305）. Conclusion There occurs Babesia microti infection in rats in part areas of Fujian Province. The positive rate was higher in wild rats than in domestic rats.

Key words: Babesia microti, Rodent, Genetic identification, Fujian

CLC Number:

R382.9

Fang-zhen XIAO, Xiu-qing Peng, Guo-ying XU, Yang CHEN, Dai-hua LIN, Yan-qin DENG. Investigation and genetic identification on Babesia infection in rodents in some areas of Fujian Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(1): 63-67.

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References 15

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鼠种 Species of rats		鼠数/只No. rodents						合计 Total
鼠种 Species of rats		尤溪 Youxi	顺昌 Shunchang	永安 Yong’an	长乐 Changle	清流 Qingliu	邵武 Shaowu	合计 Total
家鼠 Domestic rats	褐家鼠Rattus norvebicus	2	16	0	0	31	1	50
家鼠 Domestic rats	黄胸鼠R. flavipectus	0	11	0	0	8	0	19
	小家鼠Mus musculus	0	2	0	0	0	0	2
野鼠Wild rats	针毛鼠Niviventer fulvescens	19	0	12	0	0	1	32
	黄毛鼠R. losea	31	0	4	16	0	8	59
	大板齿鼠Bandicota indica	35	0	2	0	0	0	37
	社鼠R. confucianus	0	0	2	0	0	2	4
	青毛鼠R. bowersi	0	0	2	0	0	1	3
	黑线姬鼠Apodemus agrarius	0	0	0	0	0	2	2
	东方田鼠Microtus fortis	0	0	0	0	0	1	1
合计Total		87	29	22	16	39	16	209

动物种类 Different species of rats		检测数/只No. examined	阳性数/只 No. positives	阳性率/% Positive rate/%
家鼠 Domestic rats	褐家鼠 Rattus norvebicus	50	1	2.0
	黄胸鼠 R. flavipectus	19	1	-
	小家鼠 Mus musculus	2	0	0
小计Subtotal		71	2	2.8
野鼠 Wide rats	大板齿鼠 Bandicota indica	37	13	35.1
	黑线姬鼠 Apodemus agrarius	2	0	0
	黄毛鼠 R. losea	59	1	1.7
	东方田鼠 Microtus fortis	1	0	0
	青毛鼠 R. bowersi	3	0	0
	社鼠 R. confucianus	4	2	-
	针毛鼠 Niviventer fulvescens	32	2	6.3
小计Subtotal		138	18	13.0
合计Total		209	20	9.6