›› 2004, Vol. 22 ›› Issue (5): 11-296.

• 论著 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the Ribosomal DNA ITS Gene of Leishmania donovani Isolates from Hill Foci of China

TIAN Yu,CHEN Jian ping*,HU Xiao su
  

  1. Department of Parasitology,School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610044,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-10-30 Published:2004-10-30

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Abstract: 　Objective To determine the nucleotide sequence of the ITS (internal transcribed spacer) gene of Leishmania donovani isolates from hill foci (L.d.SC10 and L.d.6), and to find out the difference of the gene sequences between the two isolates. Methods Specific ITS fragments from nuclear DNA of two Leishmania isolates were amplified by PCR, cloned into pMD18 T vector, and finally sequenced by the dideoxy chain termination method. Results Sequence analysis showed that the amplified DNA fragments of the two isolates were 1 027 bp (L.d.SC10) and 1 028 bp (L.d.6) respectively, showing a sequence difference. Conclusion Sequence difference exists between the Leishmania isolates L.d.SC10 and L.d.6 from hill foci in China.

Key words: Leishmania donovani, ITS, Gene cloning, Sequence analysis