Abstract: Objective To construct and express recombinant plasmid containing the structural gene encoding Mr 31 000} antigen of Trichinella spiralis muscle larvae. MethodsThe target gene TspE1 was amplified by RT-PCR, cloned into pUC18 vector, and sub-cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3-TspE1 by gene-gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining. Results and Conclusion The recombinant plasmid pcDNA3-TspE1 was successfully constructed and expressed in the BALB/c mice.

Key words: Trichinella spiralis, DNA vaccine, antigens, gene-gun technique, eukaryotic expression