›› 2006, Vol. 24 ›› Issue (5): 21-397.

• 研究简报 • Previous Articles     Next Articles

Biological Identification on Sub-cultivation Cells ofSchistosoma japonicum Adult Worms in Vitro

LIU Wei1;ZENG Tie-bing1,4;ZENG Qing-ren1,2;CAI Chun1;ZHANG Zu-ping1;GONG Yan-fei2;CAI Li-ting2;ZHANG Shun-ke2;XU Xi-ping3   

  1. 1 Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078，China；2 Central Laboratory of Cell & Molecular Biology, Central Southern University, Changsha, 410011，China；3 Depart-ment of Ultrastructure, Xiangya School of Medicine，Changsha 410078，China；4. Institute of Pathogenic Biology, Medical College of Nanhua University, Hengyang 420001, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-30 Published:2006-10-30
  • Contact: ZENG Qing-ren

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Abstract: Cultivation of cells from 30-day old Schistosoma japonicum （S.j） adult worms showed that the growth features of the cells were semi-floating and accumulative. The survival rate of the primary cells, passage cells prior to the 5th generation and recovered cells was all up to 90%. Phases of cell division were observed during cultivation. Chromosome karyotype of the 5th generation cells possessed diploid feature of the blood-flukes（2n=8 in number）. Ultrastructure of the 5th generation cells showed that four types of cells in normal morphology and three types of cells in abnormal morphology were both viewed. It is suggested that some of the cells from S.j adult worms were subcultured successfully in the 1640-40 defined medium.

Key words: Schistosoma japonicum, Adult worm, Cell, Passage culture, Ultrastructure, Chromosome karyotype