›› 1998, Vol. 16 ›› Issue (6): 436-440.

• 论著 • Previous Articles     Next Articles

CORRELATION BETWEEN THE DOSE AND THE ANTI TOXOPLASMA EFFECT OF ACTIVATED MOUSE MACROPHAGES INDUCED BY IFN-γ AND THE SYNERGISM BETWEEN IFN-γ AND TNF-α

Zhang Aimin; Yang Huizhen; Yang Yang; Qian Zhongli   

  1. Laboratory of Parasitology; Shanghai Institute of Immunology; Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-12-31 Published:1998-12-31

PDF (PC)

Abstract: AIM: To find out the dose dependence of the anti-Toxoplasma effect induced by IFN-
γ and to determine the possible synergistic activity between TNF-α and IFN-γ. METHODS: The in
vitro effect of cultivated mouse peritoneal macrophages activated by IFN-γ alone or IFN-γ
combined with different doses of TNF-α on the intracellular tachyzoites of RH strains and the
nitric oxide (NO) level in the culture medium supernatant were simultaneously determined.
RESULTS: With the increase in the dose of IFN-γ, the anti-Toxoplasma effect was augmented and the NO level was enhanced. At 24 hours after tachyzoite invasion, a significant reversed correlation was demonstrated between the NO level and the number of intracellular parasites. CONCLUSION: The anti-Toxoplasma effect of macrophages activated by IFN-γ appears to be dose-dependent and TNF-αacts synergitically with IFN-γ in the activation of macrophages. The production of reactive NO could be an important effector in the IFN-γ primed anti-Toxoplasma action.

Key words: Toxoplasma gondii, interferon γ, tumor necrosis factor α, marcrophage, nitric oxide