›› 2010, Vol. 28 ›› Issue (2): 12-142.

• 实验研究 • Previous Articles     Next Articles

Early Kinetics of Toxoplasma gondii Infection in Mice Infected Intragastrically with Tachyzoites by Chromogenic in situ Hybridization Targeting SAG2 mRNA

MENG Xiao-li1， MA Xiao-ming1， YIN Guo-rong1 *， LIU Hong-li1，YIN Li-tian2， SHEN Jin-yan1， WANG Hailong1   

  1. 1 Institute of Medical Parasitology，Shanxi Medical University，Taiyuan 030001，China；2 Department of Physiology，Key Laboratory of Cellular Physiology Co-constructed by Province and Ministry of Education，Shanxi Medical University，Taiyuan 030001，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-30 Published:2010-04-30
  • Contact: YIN Guo-rong

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Abstract: Objective To observe the early kinetics of Toxoplasma gondii infection in mice inoculated with tachyzoites of RH strain. Methods Twenty BALB/c mice were administered intragastrically with tachyzoites of RH strain （2×104/mice）. Parasite burdens in mesenteric lymph node （MLN）, liver, spleen, lung and brain were determined by chromogenic in situ hybridization targeting SAG2 mRNA at 1, 2, 4, 6 and 8 days postinfection. Five mice were inoculated with PBS as blank control. Results The MLN, liver and spleen were the first organs where tachyzoites were found on the first day after infection, followed by the lungs on the 4th day and the brain on the 6th day. On days 6 to 8 after infection, there was a significant difference on parasite load among the tissues（P<0.05）, and the parasite load in MLN was highest, followed by that of liver, spleen, lungs and brain. The number of tachyzoites in various tissues was time-dependent. Conclusion T. gondii tachyzoites were first detected in MLN, liver and spleen, then in the lungs, and finally in the brain. The number of tachyzoites in the MLNs increased more rapidly.

Key words: Toxoplasma gondii, RNA in situ hybridization, Host tissue