›› 2006, Vol. 24 ›› Issue (1): 9-40.

• 论著 • Previous Articles     Next Articles

Cloning and Identification of the cDNA and Genomic DNA Sequences of the Defensin Gene of Anopheles sinensis

ZHANG Ya-jing,CHEN Xiao-guang,ZHENG Xue-li,WANG Chun-mei　   

  1. Department of Pathogenic Biology, Southern Medical University, Guangzhou 510515, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-02-28 Published:2006-02-28
  • Contact: CHEN Xiao-guang

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Abstract: Objective To clone and identify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene. Methods Referring to the published defenain gene sequences of Aedes aegypti and Anopheles gambiae, pairs of primers were designed to amplify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene with template of Anopheles sinensis total RNA by RT-PCR and genomic DVA by nested PCR, respectively. These amplified fractions were cloned and sequenced, and were further identified and analyzed by relevant bioinformatics softwares. Results Complete genomic DNA sequence was cloned, including 5' and 3' UTR fraction and two exons separated by a 85 bp intron. Besides, the whole cDNA sequence of Anopheles sinensis defensin gene with 324 bp was also cloned, and its ORF encoded 107 amino acids. The mature peptide had 40 amino acids residues. Conclusion The whole cDNA sequence and complete genomic DNA
sequence of the defensin gene of Anopheles sinensis have been cloned and identified for the first time.

Key words: Anopheles sinensis, Defensin: cDNA, Genomic DNA, Clone