Establishment and influencing factors of experimental animal models for intestinal protozoa infection

doi:10.12140/j.issn.1000-7423.2025.01.021

Abstract

Abstract:

Intestinal protozoa are single-celled eukaryotic microorganisms that are widely distributed in ecological environments. Most of the protozoan isolates are derived from excretions or secretions of individuals carrying cysts, which are used for experimental animal infections following purification and culture. Both the limitations of in vitro culture techniques and the host specificity of protozoa may directly affect the difficulty in the establishment of animal models. This review summarizes major in vitro culture techniques and appropriate animal models, and discusses the factors affecting the establishment of animal models. Considering that novel animal models are more effective to simulate the disease progression of human intestinal protozoan diseases, high attention should be paid to the development of experimental animal models of intestinal protozoan infections, notably novel animal models, so as to provide supports for the establishment and research of animal models of intestinal protozoan infections.

Key words: Intestinal protozoa, In vitro culture, Inoculation, Animal infection, Animal model

CLC Number:

R531

ZHANG Xiaocheng, SHEN Yujuan, HU Yuan, JIANG Yanyan, LIU Hua, CAO Jianping. Establishment and influencing factors of experimental animal models for intestinal protozoa infection[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 135-139.

Figures/Tables 1

References 42

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原虫种类	虫种来源	动物选择	动物接种技术	文献
蓝氏贾第鞭毛虫	自感染贾第鞭毛虫患者或感染动物的粪便中分离培养获得包囊	家犬、家猫、家兔、B细胞缺陷小鼠、C57BL/6N小鼠、C57BL/6J小鼠、长爪沙鼠、蒙古沙鼠等	胃部灌注	[5,18,26 -27]
溶组织内阿米巴	自然感染溶组织内阿米巴患者或感染动物的粪便中分离培养获得虫体	狗、猫、猴、兔、豚鼠、小鼠、金黄地鼠、仓鼠、长爪沙鼠等	盲肠内接种；胃部灌注	[6-7,20⇓⇓-23]
人芽囊原虫	自然感染人芽囊原虫患者或感染动物的粪便中分离培养获得虫体	大鼠、小鼠、豚鼠、猪和兔等	胃部灌注	[8]
隐孢子虫	自然感染隐孢子虫患者或感染动物的粪便中分离培养获得卵囊	大鼠、小鼠、豚鼠、犬、牛、羊、猪、鸡等	小型动物：胃部灌注大型动物：经口感染	[9-10,19]
微孢子虫	自然感染微孢子虫患者或感染动物的粪便中分离培养获得孢子	BALB/c小鼠、C57BL/6J小鼠、SCID小鼠等	腹膜内注射；直肠灌入	[11⇓⇓⇓⇓⇓-17]