Intermediate host population and infection status of Paragonimus in southeastern Youxi County, Fujian Province

doi:10.12140/j.issn.1000-7423.2021.05.022

Abstract

Abstract:

From 2015 to 2020, four villages in southeast Youxi County, Fujian Province (Puning Village and Xiwei Village in Xiwei Town, Chimu Village and Zhujian Village in Tangchuan Town) were selected as survey sites. Freshwater snails and crabs were collected in one shallow and one deep gully from each village and were identified by morphological characteristic. Paragonimus cercariae from snails and cysticerci from crabs were isolated and identified. Two puppies were fed with 80 metacercariae of P. westermani or P. skrjabini isolated from crabs. The eggs were examined in feces starting 60 days after infection, and the puppies were dissected to collect adult worms at 80 days and 100 days after infection. The adult worms were identified by morphological characteristics. We collected 7 405 freshwater snails from three species: Semisulcospira liberlina, the first intermediate host of P. westermani, and undefined Triculini and Erhaia, the first intermediate host of P. skriabini. The infection rates of P. skrjabini cercariae in Puning Village (Triculini), Xiwei Village (Triculini) and Chimu Village (Erhaia) were 0.39% (6/1 546), 0.17% (2/1 179) and 0.26% (5/1 898), respectively. The infection rate of P. westermani cercariae in S. liberlina in Zhujian Village was 0.11% (1/917). A total of 319 crabs from five species （S. fukienense, B. yonganense, B. youxiense, N. fujianense, and undefined Huananpotamon） were collected. All species were the second intermediate hosts of Paragonimus. The total infection rate of freshwater crabs was 25.71% (82/319). The average infection intensity was 7.34 metacercariae per crab, and the infection index was 3.09. The infection rate of P. skrjabini metacercariae in B. youxiense and S. fukienense in Puning Village was 41.89% (31/74). The infection rate of P. skrjabini metacercariae in undefined Huananpotamon and S. fukienense in Xiwei Village was 22.67% (17/75). The infection rate of P. skrjabini metacercariae in S. fukienense, B. yonganense, and N. fujianense in Chimu Village was 30.0% (21/70). The infection rate of P. westermani metacercariae in S. fukienense, B. yonganense, and N. fujianense in Zhujian Village was 13.00% (13/100). A total of 12 and 10 adult worms of P. westermani and P. skrjabini, respectively, were collected from puppies infected with metacercariae, respectively. Many species of Paragonimus first intermediate host snails and Paragonimus second intermediate host crabs were found in the southeast Youxi County, Fujian Province, which is a moderate-risk area for Paragonimus infection.

Key words: Paragonimus, Intermediate host, Snail, Crab, Infection rate, Youxi County

CLC Number:

R532.22

LUO Jun, CHEN Ji-qing, JIANG Dian-wei, ZHOU Pei-cong, CAI Mao-rong, CHENG You-zhu. Intermediate host population and infection status of Paragonimus in southeastern Youxi County, Fujian Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(5): 646-651.

Figures/Tables 7

References 20

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调查点	孳生地水环境类型	第一中间宿主螺类		第二中间宿主蟹类
调查点	孳生地水环境类型	螺种	感染率/%	蟹种	感染率/%	平均囊蚴数/ 只·蟹^-1	每克蟹囊蚴数/只·（g蟹）^-1
埔宁A	山垅田灌溉沟水源坑沟	待定种拟钉螺	0.39（6/1 546）	尤溪博特溪蟹	64.58（31/48）	6.45	3.98
埔宁B	华兰溪支流溪沟	放逸短沟蜷	0（0/617）	福建华溪蟹	0（0/26）
溪尾A	山坳梯田源头小沟	待定种拟钉螺	0.17（2/1 179）	待定种华南溪蟹	38.71（12/31）	6.33	2.14
				福建华溪蟹	25.00（5/20）	10.80	0.76
溪尾B	华兰溪支流溪沟	放逸短沟蜷	0（0/526）	福建华溪蟹	0（0/24）
赤墓A	山坳梯田灌溉沟源头渗水沟	待定种洱海螺	0.26（5/1 898）	永安博特溪蟹	40.17（9/23）	5.56	0.94
				福建南海溪蟹	44.44（12/27）	5.92	0.73
赤墓B	华兰溪支流溪沟	放逸短沟蜷	0（0/722）	福建华溪蟹	0（0/20）
珠建A	山涧坑沟	放逸短沟蜷	未检测	福建华溪蟹	0（0/25）
				福建南海溪蟹	0（0/20）
				永安博特溪蟹	0（0/21）
珠建B	华兰溪支流溪沟	放逸短沟蜷	0.11（1/917）	福建华溪蟹	38.24（13/34）	11.62	2.52