Laboratory detection of parasitic infections in clinical samples from hospitals in Shanghai during 2014-2017

Abstract

Abstract:

Objective To analyze the results of laboratory detection of parasitic infections in clinical samples from hospitals in Shanghai during 2014-2017. Methods Samples included in this study were from hospitals in China which cooperated with National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, consisting of 7 secondary hospitals, 28 tertiary hospitals, and 6 foreign-related hospitals. The samples included whole blood, serum, feces, sputum, urine, body fluids (hydrothorax and ascites), histopathological sections, and worms. Information of the patient’s clinical data was also recorded. Parasitic infection in the samples was examined using direct smear method, modified acid-fast staining method, ELISA, and molecular biological methods. Results A total of 25 549 clinical samples were sent to the institute for examination, including 8 877 samples for pathogen detection, of which 633 (7.1%) were found to be infected with 33 species of parasites. Specifically, 331 samples were with Blastocystis homini (3.7%) and 120 with Entamoeba histolytica (1.4%). In addition, 16 672 samples were examined by the serological method, resulting in positive results for 1 881(10.9%) samples, consisting of 424 samples seropositive for Cysticercus cellulosae (23.4%), 388 for Sparganum mansoni (21.4%), 289 for Schistosoma japonicum (16.0%), 267 for Paragonimus westermani (14.7%), and 260 for Toxoplasma gondii (14.4%). Protozoan infection was detected in 371 samples, most of which were from Shanghai (125 samples), and helminth infection was revealed by ELISA in 87 samples, mainly from Shanghai (29 samples). Conclusion B. hominis and E. histolytica were the major intestinal protozoans in pathogen detection in the samples, and the seropositive cases mainly show possible infection with C. cellulosae and S. mansoni.

Key words: Clinical samples, Parasite detection, Analysis, Shanghai

CLC Number:

R383

Peng SONG, Hao LI, Jian GUO, Lin AI, Yan LU, Yu-chun CAI, Mu-xin CHEN, Hai-mo SHEN, Shao-hong CHEN. Laboratory detection of parasitic infections in clinical samples from hospitals in Shanghai during 2014-2017[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(5): 489-494.

Figures/Tables 3

Table 1

Table 2

Different infection rates by gender and age in the samples from hospitals in Shanghai during 2014-2017

虫种 Species	总阳性率/% Overall seroprevalence/%	不同性别检出率/% Infection rate by sex/%		不同年龄组检出率/% Infection rate by age/%
虫种 Species	总阳性率/% Overall seroprevalence/%	男性 Male	女性 Female	< 20岁 < 20 years	20~30岁 20~30 years	30~50岁 30~50 years	> 50岁 > 50 years
人芽囊原虫 Blastocystis hominis	3.73(331/8 877)	3.52(192/5 462)	4.07(139/3 415)	4.19(125/2 984)	4.22(57/1 352)	3.17(86/2 717)	3.45(63/1 824)
溶组织内阿米巴 Entamoeba histolytica	1.35(120/8 877)	1.15(63/5 462)	1.67(57/3 415)	1.31(39/2 984)	1.63(22/1 352)	1.18(32/2 717)	1.48(27/1 824)
蓝氏贾第鞭毛虫 Giardia lamblia	0.18(16/8 877)	0.15(8/5 462)	0.23(8/3 415)	0.17(5/2 984)	0.37(5/1 352)	0.22(6/2 717)	0
恶性疟原虫 Plasmodium falciparum	0.27(24/8 877)	0.33(18/5 462)	0.18(6/3 415)	0.10(3/2 984)	0.44(6/1 352)	0.55(15/2 717)	0
间日疟原虫 Plasmodium vivax	0.01(1/8 877)	0.02(1/5 462)	0	0	0	0.04(1/2 717)	0
三日疟原虫 Plasmodium malariae	0.02(2/8 877)	0.02(1/5 462)	0.03(1/3 415)	0	0.07(1/1 352)	0	0.05(1/1 824)
卵形疟原虫 Plasmodium ovale	0.01(1/8 877)	0.02(1/5 462)	0	0	0	0.04(1/2 717)	0
杜氏利什曼原虫 Leishmania donovani	0.10(9/8 877)	0.07(4/5 462)	0.15(5/3 415)	0.17(5/2 984)	0	0.15(4/2 717)	0
蛔虫 Ascaris lumbricoides	0.07(6/8 877)	0.05（3/5 462）	0.09(3/3 415)	0.10(3/2 984)	0.07(1/1 352)	0.07(2/2 717)	0
人鞭虫 Trichuris trichiura	0.02(2/8 877)	0	0.06(2/3 415)	0.03(1/2 984)	0.07(1/1 352)	0	0
人蛲虫 Enterobius vermicularis	0.20(18/8 877)	0.15（8/5 462）	0.29(10/3 415)	0.5(15/2 984)	0	0.11(3/2 717)	0
钩虫 Hookworm	0.19(17/8 877)	0.15（8/5 462）	0.26(9/3 415)	0	0.07(1/1 352)	0.22(6/2 717)	0.55(10/1 824)
日本血吸虫 Schistosoma japonicum	0.06(5/8 877)	0.09（5/5 462）	0	0.07(2/2 984)	0.15(2/1 352)	0	0.05(1/1 824)
埃及血吸虫 Schistosoma haematobium	0.01(1/8 877)	0.02(1/5 462)	0	0	0	0.04(1/2 717)	0
华支睾吸虫 Clonorchis sinensis	0.08(7/8 877)	0.05（3/5 462）	0.12(4/3 415)	0	0.22(3/1 352)	0.11(3/2 717)	0.05(1/1 824)
片形吸虫 Fasciola sp.	0.05(4/8 877)	0.02(1/5 462)	0.09(3/3 415)	0	0	0.15(4/2 717)	0
牛带绦虫 Taenia saginata	0.19(17/8 877)	0.22（12/5 462）	0.15(5/3 415)	0	0.59(8/1 352)	0.29(8/2 717)	0.05(1/1 824)
阔节双叶槽绦虫 Diphyllobothrium latum	0.05(4/8 877)	0.04（2/5 462）	0.06(2/3 415)	0	0.15(2/1 352)	0	0.11(2/1 824)
日本海双叶槽绦虫 Diphyllobothrium nihonkaiense	0.11（10/8 877）	0.13（7/5 462）	0.09(3/3 415)	0	0.07(1/1 352)	0.33(9/2 717)	0
短膜壳绦虫 Hymenolepis nana	0.01(1/8 877)	0.02(1/5 462)	0	0.03(1/2 984)	0	0	0
盘尾丝虫 Onchocerca volvulus	0.01(1/8 877)	0.02(1/5 462)	0	0	0.07(1/1 352)	0	0
结膜吸吮线虫 Thelazia callipaeda	0.05(4/8 877)	0.02(1/5 462)	0.09(3/3 415)	0.03(1/2 984)	0.07(1/1 352)	0.04(1/2 717)	0.05(1/1 824)
猪囊尾蚴 Cysticercus cellulosae	0.03(3/8 877)	0.02(1/5 462)	0.06(2/3 415)	0	0	0.11(3/2 717)	0
曼氏迭宫绦虫 Spirometra mansoni	0.03(3/8 877)	0.04（2/5 462）	0.03(1/3 415)	0.03(1/2 984)	0	0.07(2/2 717)	0
粪类圆线虫 Strongyloides stercoralis	0.06(5/8 877)	0.02(1/5 462)	0.12(4/3 415)	0	0	0.18(5/2 717)	0
铁线虫 Goridus sp.	0.01(1/8 877)	0.02(1/5 462)	0	0	0	0.04(1/2 717)	0
舌形虫 Linguatula sp.	0.03(3/8 877)	0.04（2/5 462）	0.03(1/3 415)	0	0	0	0.16(3/1 824)
蠕形螨 Demodex sp.	0.03(3/8 877)	0.04（2/5 462）	0.03(1/3 415)	0.07(2/2 984)	0	0.04(1/2 717)	0
蝇蛆 Larva of Muscidae sp.	0.02(2/8 877)	0	0.06(2/3 415)	0	0.15(2/1 352)	0	0
硬蜱幼虫 Larva of lxodes sp.	0.02(2/8 877)	0	0.06(2/3 415)	0.07(2/2 984)	0	0	0
蠊缨滴虫 Lophomonas blattarum	0.02(2/8 877)	0.02(1/5 462)	0.03(1/3 415)	0	0	0.04(1/2 717)	0.05(1/1 824)
体虱 Pediculus sp.	0.01(1/8 877)	0.02(1/5 462)	0	0.03(1/2 984)	0	0	0
阴虱 Pthirus sp.	0.01(1/8 877)	0.02(1/5 462)	0	0	0	0.04(1/2 717)	0
毛蠓幼虫 Punkie larva	0.07(6/8 877)	0.09(5/5 462)	0.03(1/3 415)	0.03(1/2 984)	0.07(1/1 352)	0.15(4/2 717)	0
合计 Total	7.13(633/8 877)	6.55（358/5 462）	8.05（275/3415）	6.94（207/2 984）	8.51（115/1 352）	7.36（200/2 717）	6.09（111/1 824）

Table 2

Table 3

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虫种 Species	总阳性数 No. positives	百分比/% Proportion/%	2014		2015		2016		2017
虫种 Species	总阳性数 No. positives	百分比/% Proportion/%	阳性数 No. positives	百分比/% Proportion/%	阳性数 No. positives	百分比/% Proportion/%	阳性数 No. positives	百分比/% Proportion/%	阳性数 No. positives	百分比/% Proportion/%
猪囊尾蚴 Cysticercus cellulosae	424	23.41	158	26.78	98	21.83	107	24.43	61	18.26
日本血吸虫 Schistosoma japonicum	289	15.96	118	20.00	96	21.38	34	7.76	41	12.28
卫氏并殖吸虫 Paragonimus westermani	267	14.74	70	11.86	43	9.58	75	17.12	79	23.65
刚地弓形虫 Toxoplasma gondii	260	14.36	75	12.71	74	16.48	54	12.33	57	17.07
曼氏裂头蚴 Sparganum mansoni	388	21.42	114	19.32	95	21.16	133	30.37	46	13.77
细粒棘球绦虫 Echinococcus granulosus	86	4.75	28	4.75	27	6.01	14	3.20	17	5.09
片形吸虫 Fasciola sp.	2	0.11	1	0.17	0	0	0	0	1	0.30
华支睾吸虫 Clonorchis sinensis	78	4.31	20	3.39	11	2.45	18	4.11	29	8.68
旋毛虫 Trichinella spiralis	12	0.66	6	1.02	4	0.89	1	0.23	1	0.30
广州管圆线虫 Angiostrongylus cantonensis	3	0.17	0	0	1	0.22	0	0	2	0.60
尖吻蝮蛇舌状虫 Armilliger agkistrodontis	2	0.11	0	0	0	0	2	0.46	0	0
合计 Total	1 811	100	590	100	449	100	438	100	334	100