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Preliminary Analysis of the Genetic Loci of SAG1 and SAG3, the Surface Antigens of Toxoplasma gondii, in HIV-positive People in Dali of Yunnan Province

LENG Li1,2, CHEN Ling-juan3,4, LI Wei3, HE Yan-hong3,LUO Mi5, GAO Ju6, MA Ning1, SHEN Li-jie1 *   

  1. 1 Department of Pathogen Biology and Immunology, Kunming Medical University, Yunnan Province, Kunming 650500, China; 2 Clinical Laboratory of the Second Pepole’s Hospital in Qujing, Qujing 655000, China；3 Dali University, Dali 671000, China; 4  Clinical Laboratory of the Pepole’s Hospital in Yangjiang City of Guangdong Province，Yangjiang 529500, China; 5 The First Clinical College of Chongqing Medical University, Chongqing 400042, China; 6 Clinical Laboratory of the Second Pepole’s Hospital in Dali City, Dali 671000, China
  • Online:2015-08-30 Published:2015-09-10

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Abstract:

Objective  To identify the genotypes of Toxoplasma gondii that infects HIV-positive people in Dali of Yunnan Province through analyzing the genetic loci of the surface antigens SAG1 and SAG3.  Methods  A total of 291 blood samples from HIV-positive cases were collected from the HIV/AIDS Prevention and Control Institution in Yunnan. Nested PCR was used to amplify SAG1 and SAG3 genes in the blood samples. The products were digested with restriction enzymes Sau96Ⅰ, HaeⅡ and NciⅠ, and sequenced.  Results  Of the 291 HIV-positive blood samples, 64 showed successful amplification of SAG1 gene, and 42 of SAG3 gene, with product sizes of 390 bp and 225 bp, respectively. Enzymetic digestion of the PCR products resulted in fragments of 350 bp and 50 bp for SAG1, and -200 bp band for SAG3, consistent with RH, a particular type Ⅰ strain of T. gondii. Sequencing of the SAG1 and SAG3 PCR products showed that their sequence identities with SAG1 （Accession No. GQ253073） and SAG3（Accession No. JX218225.1） of the type Ⅰ strain of T. gondii were 99.98%-100% and 99.96%-99.98% respectively.  Conclusion  The Toxoplasma gondii in HIV-positive cases in Dali of Yunnan Province is the type I strain of T. gondii.

Key words: Toxoplasma gondii, HIV, SAG1, SAG3, Genotype