Abstract: One hundred and one imported falciparum malaria cases in Wuhan City were confirmed by microscopy and Nest-PCR， and the blood samples were collected. The Pf60.1 gene was amplified by PCR. Among 101 blood samples， three Pf60.1 fragments ［313 bp （56.5%， 52/92）， 340 bp （37.0%， 34/92）， 313 bp+340 bp （6.5%， 6/92）］ were amplified from 92 samples. Among 83 blood samples from patients returning from Africa， 313 bp fragment were found in 46 samples （55.4%， 46/83）， 340 bp fragment were found in 31 samples （37.1%， 31/83）， and 7.2% （6/83） was mixed-fragment （313 bp+340 bp）. Among 9 samples from southeast Asia， 6 samples were with 313 bp fragment and 3 samples with 340 bp fragment. The results indicated that the most common genotype was 313 bp-genotype， and there would be polyclonal P. falciparum infections.

Key words: Imported case, Plasmodium falciparum, Pf60.1, Polymorphism