Total Protein Analysis by Two-dimensional Electrophoresis in Cysticerci of Taenia solium and Taenia asiatica

Abstract

Abstract: Two 20-day-old three-way crossed hybrid pigs were infected with 80 000 Taenia solium or T. asiatica eggs, respectively. Immature cysticerci of the two species in liver were collected at 40 days after infection. The total proteins were separated by two-dimensional electrophoresis, and differentially expressed proteins were analyzed by ImageMaster 2D Plantinum 6.0 software. The results showed that there were （236±12） and （231±14） protein spots in 2D electrophoresis gel images of T. solium and T. asiatica, respectively, with 3 proteins up-regulated and 7 proteins down-regulated in T. solium cysticercus by 2-fold or more compared with those in T. asiatica cysticercus.

Key words: Taenia solium, Taenia asiatica, Cysticercus, Two-dimensional electrophoresis, Proteome

FANG Wen, XIAO Liang-Liang, BAO Huai-En, MU Rong. Total Protein Analysis by Two-dimensional Electrophoresis in Cysticerci of Taenia solium and Taenia asiatica[J]. , 2011, 29(3): 18-188-190.

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Total Protein Analysis by Two-dimensional Electrophoresis in Cysticerci of Taenia solium and Taenia asiatica

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