›› 2011, Vol. 29 ›› Issue (3): 17-176-178.

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Laboratory Diagnosis of HIV/AIDS Patients Complicated with Pneumocystis jirovecii Poneumonia

CHEN  Jing-Jie, LI  Yong, HE  Han, SU  Ling-Song, QIN  Song-Shu, HU  Hong-Bo, TANG  Liu-Sheng, LI  Shi-Li   

  1. 1 Department of Clinical Laboratory，Longtan Hospital，Liuzhou 545005，China；2 Department of HIV/AIDS，Longtan Hospital，Liuzhou 545005，China；3 Department of Clinical Laboratory，Tumor Hospital of Liuzhou City，Liuzhou 545002，China；4 Department of Clinical Laboratory，Liuzhou Railway Center Hospital，Liuzhou 545006，China
  • Online:2011-06-30 Published:2012-09-27

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Abstract: Pneumocystis jirovecii was detected in sputum samples and bronchoalveolar lavage fluid （BALF） obtained from HIV/AIDS patients complicated with Pneumocystis jirovecii poneumonia by Giemsa staining. CD4+ T lymphocytes of 500 patients were counted by flow cytometer. P. jirovecii positive rate in sputum samples （46.8%， 845/1 806） was significantly lower than that of BALF （55.8%， 106/190）（P<0.05）. The proportion of patients developing clinical symptoms in P. jirovecii positive cases （96.6%， 816/845） was higher than that of P. jirovecii negative cases （64.0%， 615/961）（P<0.05）. P. jirovecii positive rate increased with the decrease of CD4+ T lymphocyte number. P. jirovecii positive rates in cases with CD4+ ＞200×106/L， CD4+ 200×106/L-100×106/L， and CD4+＜100×106/L were 12.0%（6/50）, 39.0%（39/100）, and 54.6% （191/350）, respectively （P＜0.05）. Giemsa staining is an efficient, simple and feasible method for P. jirovecii detection, relying on the experience and skill of the operator.

Key words: AIDS, Pneumocystis jirovecii, Giemsa staining, BALF, CD4+lymphocyte