Abstract: Objective   To investigate the therapeutic efficacy of ginsenoside Rg3 on hepatic fibrosis in murine schistosomiasis japonica.  Methods   54 ICR-strain male mice were divided into 4 groups named as normal control group （A）, infected control group （B）, praziquantel+Rg3 treated group （C） and praziquantel treated group （D）. There were 12 mice in each group, but 18 in group A. Mice in groups B，C，and D were infected with 20±2 cercariae of Schistosoma japonicum. At ten weeks post-infection, 10 mice of group A and 12 mice of group B were weighed and  sacrificed. Specimens from left hepatic lobes were taken and fixed in 10% formaldehyde solution. Mice in groups C and D were treated intragastrically with praziquantel at a single dose of 300 mg/kg. At the second day after praziquantel treatment, each mouse in group C was given 3 mg/（kg·d） ginsenoside Rg3 for 8 weeks. The rest mice were sacrificed on 8 weeks after treatment, and liver tissue samples from left hepatic lobes were prepared. The histological changes and collagen fiber deposition in the liver tissue sections were observed with hematoxylin-eosin staining and van gieson staining. Liver fibrosis was graded according to semi-quantitative scoring system （SSS） method.  Results  In group B, many eggs deposited in the hepatic lobules and portal areas, and eosinophilic abscesses and pseudo-tubercles developed in the liver， especially common in portal areas. There were many fibre hyperplasia and deposit inside abbacy and liver flocculus. Pipestem fibrosis formed around the portal areas, and some cord-like fibres extended into hepatic lobules, and formed in the fibrous septa. After 8-week treatment with ginsenoside Rg3, in group C, the livers were initially enlarged, firm and dust-color; and the degree of hepatomegly varied from mild to marked; but the degree of fibre hyperplasia and inflammatory infiltration were mitigated compared with that of group B. Mean percentage of collagen area in group C［（2.32±0.99）%］ was lower than that of groups B ［（11.08±4.43）%］ and D ［（11.19±4.91）%］（P<0.05）. The SSS scores of hepatic fibrosis in group C （2.83±1.09） was lower than that of groups B （7.42±1.16） and D（8.08±1.76）（P<0.05）.  Conclusion   Ginsenoside Rg3 shows anti-hepatofibrosis effects in murine schistosomiasis japonica after praziquantel treatment.

Key words: Schistosomiasis japonica, Hepatic fibrosis, Ginsenoside Rg3