›› 2011, Vol. 29 ›› Issue (2): 11-134-137.

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Investigation on Malaria Vectors in Western Part of Cina-yanmar Border

SHI Wen-qi1，ZHOU Xiao-jun1，ZHANG Yi1 *，ZHOU Xiao-nong1，HU Ling1，WANG Xue-zhong2，WANG Jian2，LI Yan-jun3   

  1. 1 National Institute of Parasitic Diseases，Chinese Center for Disease Control and Prevention；Key Laboratory of Parasite and Vector Biology，MOH；WHO Collaborating Centre for Malaria，Schistosomiasis and Filariasis，Shanghai 200025，China；2 Yunnan Institute of Parasitic Diseases，Puer 665000，China；3 Institute of Pathogens and Vectors, Dali University, Dali 671000, China
  • Online:2011-04-30 Published:2012-09-27

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Abstract: Objective   To reveal the distribution and composition of malaria-ransmitting vectors on the western part of China-yanmar border.  Methods   An entomological survey of malaria vectors was carried out in six villages of Yingjiang County and Xidong County on China-yanmar border between August and September， 2008. Mosquitoes in human dwellings and cattle sheds were collected by overnight trapping with ovitrap light. The mosquitoes were firstly identified morphologically, and then Anopheles minimus A and C，An. aconitus，and An. jeyporiensis were identified by using multiplex PCR. Some mosquitoes were selected to extract the total genomic DNA, and detect sporozoites by nested PCR.   Results  A total of 4 571 mosquitoes were captured with 54.32% （2 483/4 571） of anopheline mosquitoes. There was significant difference in Anopheles species composition in human dwellings and cattle sheds. The main species in human dwellings were An. kochi，An. minimus，and An. sinensis， while the principal species in cattle sheds consist of An. kochi（223）， An. annularis（184）， An. vagus（131），and An. jeyporiensis（129）. Furthermore，the composition in human dwellings of villages with and without cattle was significantly different. An. minimus（260） and An. kochi（49） were the most important species in villages with cattle， whereas An. kochi（481） and An. sinensis（124） were the key species in villages without cattle. A total of 1 075 mosquitoes were examined for sporozoites and 9 mosquitoes were found to be infected. Only three species， i.e. An. minimus （7/408）， An. aconitus （1/125） and An. pseudowillmori（1/101） were infected with malaria parasite. All sporozoites were identified as Plasmodium falciparum by  sequencing， the target fragment was 204 bp.  Conclusion   The species composition of mosquitoes is complex in the study sites on the western part of China-yanmar border, and An. minimus is the major malaria vector. Additionally， An. aconitus and An. pseudowillmori are also confirmed as potential malaria vector in this area.

Key words: Malaria vector, Multiplex PCR, Nested-CR, Sporozoite, China-yanmar border