Abstract: Objective To identify and purify the allergens of Tyrophagus putrescentiae. Methods Tyrophagus putrescentiae extract was analyzed by SDS-PAGE and Western blotting， and was partially purified by DE52 anion-exchange chromatography and HiLoad 16， 60 Superdex 200 prep grade Size-exclusion chromatography. Results 23 bands were found after SDS-PAGE with comparative molecular weight （Mr） of 177 000， 118 000， 107 000， 70 000， 67 000， 60 000， 52 000， 45 000， 41 000， 40 000， 38 000， 37 000， 35 000， 27 000， 23 000， 22 000， 18 000， 17 000， 16 000， 15 000， 14 000， 13 00 and 12 000. Five allergens were detected by Western blotting with Mr 128 000， 67 000-70 000， 36 000-37 000， 18 000 and 16 000， respectively. The positive reaction rate of 3 allergens， with Mr 128 000， 67 000-70 000 and 36 000-37 000， were 100%， while that of other 2 allergens with Mr 18 000 and 16 000 was 77.8% and 44.4% respectively。The allergen with Mr 18 000 was purified by anion-exchange chromatography and Size-exclusion chromatography. Conclusion Four major allergens and one minor allergen from Tyrophagus putrescentiae have been identified.

Key words: Tyrophagus putrescentiae, Allergen, Size-exclusion chromatography