›› 2007, Vol. 25 ›› Issue (2): 5-105.

• 论著 • Previous Articles     Next Articles

Effect of Different Adjuvant Formulations on the Induced Protection of Mice Immunized With Recombinant Protein Ts87 of Trichinella spiralis

LI Qiang;YANG Jing;YANG Ya-ping;GU Yuan;LIU Zhi-ying;HUANG Song;ZHU Xin-ping   

  1. Department of Parasitology,Capital Medical University,Beijing 100069,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-04-30 Published:2007-04-30
  • Contact: ZHU Xin-ping

Abstract:

Objective To study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant Ts87 protein (rTs87) of Trichinella spiralis. Methods ICR mice were vaccinated subcutaneously 3 times in 2-week intervals with rTs87 and different adjuvants CFA or IFA, Montanide IMS1312, Montanide ISA720, Quil-A and Al(OH)3, respectively. The antibody response was detected by ELISA and the purified rTs87 was analyzed by SDS-PAGE and Western blot. The protection induced in mice was then evaluated by muscle larvae reduction after Trichinella spiralis challenge. Results The results clearly showed that all the 6 adjuvants were able to enhance specific anti-rTs87 IgG response. SDS-PAGE and Western blot revealed that the molecular weight of expressed protein was around Mr 40 000 and could be recognized by sera from mice infected with Trichinella spiralis and mice immunized with rTs87. The reduction rate of muscle larvae was 49.4%, 49.2%, 63.5%, 65.1% and 70.8%, respectively. Conclusion Protective immunity of rTs87 can be enhanced by adjuvant CFA or IFA, IMS1312, ISA720, Quil-A and Al(OH)3, and better effect is observed with the latter 3 adjuvants.

Key words: Trichinella spiralis, Recombinant protein, Adjuvant, Protection