Cloning and Expression of Aldolase Encoding Gene of Plasmodium falciparum FCC1/HN Strain

›› 2004, Vol. 22 ›› Issue (3): 2-135.

• 论著 • Previous Articles Next Articles

Cloning and Expression of Aldolase Encoding Gene of Plasmodium falciparum FCC1/HN Strain

ZHANG Rui-juan,ZHU Huai-min,CAO Yi,ZHOU Ai-guo,ZHENG Zhi-qiang,ZHANG Qing-feng,ZHENG Hui

Department of Pathogenic Biology,The Second Military Medical University,Shanghai,200433,China

Received:1900-01-01 Revised:1900-01-01 Online:2004-06-30 Published:2004-06-30

Abstract

Abstract: 　Objective To clone, sequence, and express the aldolase(ALD)encoding gene of Plasmodium falciparum FCC1/HN strain. Methods The ALD encoding gene was amplified by PCR from genomic DNA of FCC1/HN strain. The positive clones were screened and identified by agarose gel electrophoresis and endonuclease. The recombinant plasmid was transformed into E. coli M15. The fusion protein was expressed by IPTG induction and purified by Ni-NTA affinity chromatography and anion exchange column. Results The ALD gene of P. falciparum was amplified. Analysis of sequencing showed that the ALD gene of P. falciparum was identical with the sequence of other reported isolates. A Mr 41 000 fusion protein was induced by IPTG and was purified by chromatography. Conclusion The ALD gene of P. falciparum FCC1/HN strain was identical to the other reported isolates. ALD fusion protein of P. falciparum was expressed and purified.

Key words: Plasmodium falciparum, Aldolase, Cloning, Sequencing, Expression

ZHANGRui-juan;ZHUHuai-min;CAOYi;ZHOUAi-guo;ZHENGZhi-qiang;ZHANGQing-feng;ZHENGHui. Cloning and Expression of Aldolase Encoding Gene of Plasmodium falciparum FCC1/HN Strain[J]. , 2004, 22(3): 2-135.

[1]	PU Na, BO Xinwen, ZHAO Wenqing, CHEN Xuke, ZHANG Yuxia, ZHANG Yanyan, SUN Yan, WANG Zhengrong. Prokaryotic expression and preliminary functional identification of Echinococcus granulosus calreticulin [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 223-230.
[2]	LI Fei, LIU Yaobao, CAO Jun. Researches progress on molecular surveillance of artemisinin resistance in Plasmodium falciparum [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 260-268.
[3]	HUANG Lihua, WU Jun, LI Zhengjin, LUO Jiao, GU Wei. Fasciola hepatica infection complicated by hepatic tuberculosis: a case report [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 304-306.
[4]	LI Qingyu, JIN Yong, MA Weiqiong, HUANG Zhiyong. A case of central nervous system and pulmonary infection caused by Angiostrongylus cantonensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 307-310.
[5]	XING Jia, YU Yanli, WANG Jianjun, XU Shijuan. A case of visceral leishmaniasis with Aspergillus niger infection in Shaanxi Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 820-822.
[6]	LI Suhua, JI Penghui, WANG Dan, WANG Hao, ZHOU Ruimin. Analysis of polymorphism of Pfhrp2 gene in imported Plasmodium falciparum in Henan Province in 2020 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(5): 664-667.
[7]	GUO Sihan, HUANG Xin’an, XU Hanli, LI Chunxiao, LIU Kangkang, XING Dan, ZHAO Teng. Analysis on DNA methylation levels in mosquitoes of the Culex pipiens complex [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 502-511.
[8]	BAI Houxi, YANG Xiaotao, LUO Yonghan, WANG Yanchun. A case of Fasciola hepatica infection combined with Pseudomonas aeruginosa in a child [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 550-553.
[9]	JIANG Nan, SU Yaxin, JIANG Xiaofeng, SHEN Yujuan, CAO Jianping. Heterogeneity analysis of T cells in liver of mice infected with Echinococcus granulosus based on single-cell RNA sequencing [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 286-294.
[10]	WU Yixuan, GUO Xiaola, CHEN Yixia. MicroRNA differential expression profiles and their diagnostic value in the sheep infected with Echinococcus granulosus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 295-302.
[11]	YAN He, HUANG Fang, FENG Jun, YIN Jianhai, XIA Zhigui, CAO Jianping. Polymorphism of sulfadoxine-pyrimethemine resistant gene of Plasmodium falciparum in China-Myanmar border area from 2010 to 2018 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 153-159.
[12]	LIU Yi, CAI Yuchun, CHEN Jiaxu, CHEN Shaohong, YU Yingfang. Isolation, identification, and omics analysis of extracellular vesicles from Trichinella spiralis newborn larvae [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 225-233.
[13]	XIE Xiaoman, SUN Hang, DAI Lisha, ZHU Wenju, WANG Lilei, XIE Huanhuan, DONG Hongjie, ZHANG Junmei, WANG Qi, ZHOU Beibei, ZHAO Guihua, XU Chao, YIN Kun. Effect of Toxoplasma gondii infection on m⁶A methylation modification of transcripts in mice brain tissue [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 27-35.
[14]	LI Qisong, YANG Li, TENG Feixiang, MA Guifang. Cloning, expression, immunogenicity and bioinformatics analysis of Dermatophagoides pteronyssinus allergen Der p 4 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 42-47.
[15]	WU Jiahui, SONG Xiao, CHENG Peng, LIU Hongmei, GUO Xiuxia, WANG Haifang, GONG Maoqing. Identification and analysis of miRNA targeting CYP450s genes in mosquitoes [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(6): 683-690.

Cloning and Expression of Aldolase Encoding Gene of Plasmodium falciparum FCC1/HN Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments