›› 2003, Vol. 21 ›› Issue (5): 13-302.

• 实验研究 • Previous Articles     Next Articles

A Rapid Procedure to Purify Serum IgG from Microtus fotis

JIANG Shou-fu,PAN Cai-e,HE Yan-yan,ZHU Min,LI Hao,SHI Yao-jun,WEI Mei-xiong   

  1. 1 Shanghai Municipal Center for Disease Control & Prevention;Shanghai 200336 2 Shanghai Institute of Veterinary Parasitology,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai 200232
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-10-30 Published:2003-10-30

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Abstract: 　Objective To evaluate the procedure to purify IgG antibodies from Microtus fotis serum. Methods IgG antibodies from sera of three groups of Microtus fotis were purified by protein G or protein A affinity chromatography, their purity and binding capacity were compared. Results The protein G affinity chromatography was more efficient than protein A affinity chromatography. The antibodies isolated from protein G affinity chromatography showed a higher purity and better activity than that from protein A affinity chromatography monitored by SDS-PAGE and ELISA. The ability of the purified IgG to bind the second antibodies were 8.5 times and 3.1 times that of non-IgG proteins and unpurified sera, respectively. Conclusion The protein G affinity chromatography is a rapid, convenient and reliable procedure for Microtus fotis serum IgG purification.

Key words: Microtus fotis, affinity chromatography, protein G, IgG