›› 2003, Vol. 21 ›› Issue (4): 5-213.

• 论著 • Previous Articles     Next Articles

Polymorphism Analysis of Cytochrome C Oxidase 1 COI Gene in Necator americanus Collected from 5 Provinces in China

LI Tie-hua,GUO Xiang-rong,HU Ling,XIAO Shu-hua,XUE Hai-chou,John Hawdon
  

  1. Institute of Parasitic Diseases,Chinese Centre for Disease Control and Prevention,WHO Collaborating Centre for Malaria,Schistosomiasis and Filariasis,Shanghai 200025 Department of Microbiology and Tropical Medicine,George Washington University Medical Center
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-30 Published:2003-08-30

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Abstract: 　Objective To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates. Methods COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced. Results The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation oc- curred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the se- quences ranged from 1. 34% to 2. 18%. Conclusion The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.

Key words: Necator americanus, cytochrome C oxidase 1, genetic diversity, sequence analysis