›› 2003, Vol. 21 ›› Issue (2): 9-95.

• 论著 • Previous Articles     Next Articles

Construction and Expression of the Recombinant Plasmid pcDNA3.1/Ts87 of Trichinella spiralis

YANG Ya-ping1,ZHU Xin-ping1*,YANG Jing1,ZHANG Lu-ping2,XU Xu-na2,HUANG Song1,Pascal Boireau3
  

  1. 1 Department of Parasitology,Capital University of Medical Sciences,Beijing 100054 2 College of Life Science of Hebei Normal University,Shijiazhuang 050016 3 UMR BIPAR INRA AFSSA ENVA UPVM,Maisons Alfort,France
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-30 Published:2003-04-30

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Abstract: 　Objective To construct and express DNA plasmid of Trichinella spiralis. Methods The BamHI and Hindlll enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNAS. 1( + ) vector with T4 ligase. The recombinant plasmid pcDNAS. 1/Ts87 and plasmid pcDNAS. 1 were transfected into an eukaryotic cell line GOS7 through Lipofectamine, respectively. The BALB/c mice were immunized with the purified plasmid DNA pcDNAS. 1/Ts87 through two routes: intramuscular injection and gene-gun injection. Results and Conclusion The pcDNAS. 1/Ts87 was expressed both in COS7 and in the BALB/c mice.

Key words: Trichinella spiralis, DNA plasmid, gene-gun, construction, expression