Abstract: 　Objective To investigate the genomic DNA difference between chloroquine sensitive (N) and chloroquine resistant (RC) strains of Plasmodium berghei . Methods By means of polymerase chain reaction(PCR), the abstracted genomic DNAs of the N and the RC strains of Plasmodium berghei were amplified with 37 random primers(RAPD) and 84 anchored primers (APAD). The PCR products were analysed by electrophoresis and statistics. Results Of 440 bands amplified with RAPD, 196 bands were common to both strains whereas 43 bands were unique to either the RC or the N strain, with a similarity coefficient (SI) of 0.89, and a distance coefficient (D) of 0.197. Similarly, among 952 bands amplified with APAD, 436 bands were shared by both strains, and 53 bands were unique to either of them with SI 0.91 and D 0.155 respectively. Conclusion The genomic DNAs between the RC and the N strains of Plasmodium berghei are highly homogeneous.

Key words: Plasmodium berghei, chloroquine resistant (RC) and sensitive strains(N), RAPD, APAD, genomic DNA