Comparative Effect of Different Media in in vitro Cultivation of Trichomonas vaginalis

›› 2002, Vol. 20 ›› Issue (1): 13-44.

• 实验报道 • Previous Articles Next Articles

Comparative Effect of Different Media in in vitro Cultivation of Trichomonas vaginalis

GENG Zhi-hui¹;HAN Shu-mei²;LIU Li³;SHI Yu-lu¹;LI Shu-hong³

1 Department of the third centre;Norman Bethune University of Medical Sciences;Changchun 130021; 2 Department of Obstetrics and Gynecology;the First Clinical Hospital;N

Received:1900-01-01 Revised:1900-01-01 Online:2002-02-28 Published:2002-02-28

Abstract

Abstract: 　Objective To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T.vaginalis. Methods An isolate of T.vaginalis from clinical specimens was cultivated in three different media with initial inoculation of 9.0×104/ml under pH 5.6. Results There was distinct difference after 96h incubation in the cumulative harvest of T.vaginalis. The highest harvest was received in cysteine/liver/peptone/maltose medium, followed by the liver/peptone/maltose medium and soybean/liver/peptone/maltose medium. Conclusion The cysteine/liver/peptone/maltose medium may be a suitable environment for in vitro multiplication of T.vaginalis.

Key words: Trichomonas vaginalis, medium, in vitro culture

GENGZhi-hui;HANShu-mei;LIULi;SHIYu-lu;LIShu-hong. Comparative Effect of Different Media in in vitro Cultivation of Trichomonas vaginalis[J]. , 2002, 20(1): 13-44.

[1]	LUO Cheng-yang, HAO Li-xia, GUO Li-hua, WANG Bing-li, JI Si-fan, LIAN An-na, ZHU Xiao-hui, MEI Xue-fang, TIAN Xiao-wei, WANG Shuai, ZHANG Zhen-chao. Affect of Trichomonas vaginalis excretory secretory protein on sperm quality of mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(2): 236-241.
[2]	DUAN Yu-juan, LI Peng-ju, SANG Yu-hui, WU Pu-cheng, SHANG Yu-jia, HAO Li-xia, WANG Shuai, LI Xiang-rui, ZHANG Zhen-chao. Affect of Trichomonas vaginalis infection on male reproductive system and its mechanisms [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(4): 532-536.
[3]	ZHAO Li-li, MIAO Xu, XIE Huan-fei, LIU Bi-han, YUAN Lin, LIN Lin, HUANG Shuang, JIANG Xu-gan, CHEN Sheng-xia, SHEN Yu-juan, CAO Jian-ping. Detection of Trichomonas vaginalis by PCR and LAMP [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(2): 260-264.
[4]	SONG Tao, LI Hai-Tao, YANG Ling-Fei, YAO Lan-Hui, WEN Hao. Contrast-Enhanced Ultrasonography of Hepatic Alveolar Echinococcosis in Rats: the Correaltion of Imaging Fratures and Histologic Microvascular Density [J]. , 2014, 32(3): 7-200-204.
[5]	WANG Pin-jia1，XIE Cheng-bin2 . Mycoplasma hominis* Symbiosis and Trichomonas vaginalis Metronidazole Resistance [J]. , 2012, 30(3): 9-210-213.
[6]	TANG Zi-Hao, XU Jing-Bo, MEI Jun, GAO Xing-Zheng. Research Progress on the Molecular Pathogenesis of Trichomonas vaginalis [J]. , 2011, 29(3): 11-215-218,223.
[7]	TANG Zi-hao，LIU Ke-yue，MEI Jun，GAO Xing-zheng. In Vitro Anti-Trichomonas vaginalis Effects of a Mixture of Dihydroartemisinin and Metronidazole [J]. , 2010, 28(6): 4-416-422.
[8]	WEN Wen-jing，LIU Xiao-dong. MGE-PCR in Analyzing the Genetic Characteristics and the Clinical Manifestation of Trichomonas vaginalis Isolates [J]. , 2010, 28(6): 7-431-434.
[9]	YUANYu-qing;XUEChang-gui. Cryopreservation of Trichomonas vaginalis* and its influence factors [J]. , 2010, 28(5): 19-396.
[10]	YUANYu-qing;XUEChang-gui. Influence of Iron Ion on the Growth of Trichomonas vaginalis In Vitro* [J]. , 2010, 28(4): 7-276.
[11]	YANYan;GAOXing-zheng. Protein Analysis of Trichomonas vaginalis Treated by Pulsatilla chinensis In vitro [J]. , 2010, 28(3): 15-233.
[12]	ZHANGXiao-xiang*;ZHENGShan-shan;ZHUMei-mei. In Vitro Effect of Secnidazole Benzoate on Trichomonas vaginalis [J]. , 2009, 27(2): 4-114.
[13]	ZHAOJian-ling;GAOXing-zheng;QUMing. Killing Effect of Polymorphonuclear Neutrophils on Trichomonas vaginalis* [J]. , 2008, 26(5): 10-369.
[14]	FANGZheng-ming;DENGWei-wen;ZHUHong-gang;YANGZhen-de;LIUJian-jun. Observation on Mitosis of Trichomonas vaginalis* Cultivated in vitro Using Modified Giemsa Staining [J]. , 2008, 26(5): 19-398.
[15]	JIAWan-zhong;LIZhi;ZHAOLiang;LUNZhao-rong*. Genetic Variation and Clustal Analysis of Trichomonas vaginalis Cysteine Proteases [J]. , 2008, 26(3): 8-196.

Comparative Effect of Different Media in in vitro Cultivation of Trichomonas vaginalis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments