›› 1999, Vol. 17 ›› Issue (3): 3-139.

• 论著 • Previous Articles     Next Articles

TAXONOMIC STUDIES ON ECHINOCHASMUS FUJIENENSIS AND ITS RELATED SPECIES BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS AND EXPERIMENTAL INFECTION

CHENG Youzhu 1 ZHANG Yaojuan 2 LIN Chenxin 1 ZHAN Zihao 2 YU Xiaozhong 2 LIN Jinxiang 1 CAI Mingjie 3 CHE Weihong 3   

  1. 1　Fujian Provincial Institute of Parasitic Discases, Fuzhou　3500012　Department of Parasitology,Nanjing Medical University,Nanjing 2100293　Sanitary and Anti-epidemic Station of Hanchuan City , Hubei Province, H anchuan　432300
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-06-30 Published:1999-06-30

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Abstract: 　AIM:To explore the identification and differentiation of Echinochasmus fujianensis,Echinochasmus japonicus(Jiangxi strain and Fujian strain), Echinochasmus liliputanus Anhui and Echinochasmus perfoliatus Hubei.METHODS:Random amplified polymorphic DNA analysis (RAPD) and experimental animal infection were performed.RESULTS:469 polymorphic DNA fragments were obtained by 28 primers from 4 Echinochasmus species and strains in Fujian,Anhui and Jiangxi.20.8% and 97.6% of the fragments in Echinochasmus fujianensis were the same as those in Echinochasmus japonicus Fujian strain and in Echinochasmus liliputanus Anhui, respectively,99.7% of the fragments were the same between Echinochasmus japonicus Jiangxi strain and Fujian strain. CONCLUSION: Echinochasmus fujianensis and Echinochasmus liliputanus Anhui are the same species.Echinochasmus fujienensis is an independent species different from Echinochasmus japonicus.Polyinfection of Echinochasmus fujianensis,Echinochasmus japonicus and Echinochasmus perfoliatus exist in all the 3 provinces,Hubei,Anbui and Fujian,of which Echinochasmus fujienensis is a dominant species.

Key words: Echinochasmus fujianensis, random amplified polymorphic DNA, experimental infections, species