Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia

doi:10.12140/j.issn.1000-7423.2025.04.022

CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2025, Vol. 43 ›› Issue (4): 592-595.doi: 10.12140/j.issn.1000-7423.2025.04.022

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Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia

LI Jian¹^,²^,³()(), LI Fenqi², GAO Nan⁴, LIU Qiang⁵, ZHOU Qing’an⁶, LI Chunfu², HU Wei²^,³^,^*()()

1 College of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China
2 School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China
3 School of Life Sciences, Fudan University, Shanghai 200438, China
4 Hohhot Wildlife and Plant Protection Association, Hohhot 010021, Inner Mongolia, China
5 Hohhot Landscaping Construction Service Center, Hohhot 010021, Inner Mongolia, China
6 Guangxi Center for Animal Disease Control and Prevention, Nanning 530200, Guangxi, China

Received:2025-01-23 Revised:2025-07-06 Online:2025-08-30 Published:2025-10-09
Contact: *E-mail: huw@fudan.edu.cn
Supported by:
National Parasitic Resources Center, the Ministry of Science and Technology Fund(NPRC-2019-194-30);National Natural Science Foundation of China(U22A20526)

Abstract

Abstract:

Trematodes were collected from the pharynxes and oesophagi of Ciconia nigra at the Daqingshan Wildlife Park in Hohhot, Inner Mongolia on August 2024. Ten morphologically intact adult worms were sampled and stained with hydrochloric carmine for morphological observation. Genomic DNA was extracted from three adult worms, and the internal transcribed spacer 2 (ITS2) gene was amplified using PCR assay and sequenced. ITS2 gene sequences were aligned using BLAST in GenBank and a phylogenetic tree was built using the software MEGA 11.0 with the neighbor-joining method. A total of 157 trematodes were collected from pharynxes of 10 C. nigra, with mean infection intensity of approximately 16 (range, 8 to 33) worms per bird. The trematodes appeared a leaf-like shape, with dendroidal testes and anterior ovary, which matched the morphological characteristics of Cathaemasia hians. PCR assay and paired-end sequencing yielded 3 ITS2 gene sequences, with 425 bp in length, which was identical to the ITS2 sequence of C. hians. The ITS2 sequence had been deposited in GenBank under accession number PQ899708. Sequence alignment showed that the ITS2 gene sequence had a single C→T substitution at position 365 with the Czech isolate of C. hians, and phylogenetic analysis revealed that the ITS2 gene sequence was clustered with the reference sequence of C. hians in GenBank into the same clade (98% bootstrap value), indicating that the ITS2 gene sequence had an intra-species monophyly and a relatively stable genetic background.

Key words: Cathaemasia hians, Ciconia nigra, Internal transcribed spacer 2, Phylogenetic analysis

CLC Number:

R383.2

LI Jian, LI Fenqi, GAO Nan, LIU Qiang, ZHOU Qing’an, LI Chunfu, HU Wei. Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(4): 592-595.

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[1]	许秀, 吴逸群. 黑鹳的生态生物学研究与保护[J]. 湖北农业科学, 2013, 52(13): 3086-3088, 3094.
	Xu X, Wu YQ. Study on protection and eco-biology of Ciconia nigra[J]. Hubei Agric Sci, 2013, 52(13): 3086-3088, 3094. (in Chinese)
[2]	Ramilo DW, Caetano I, Brazio E, et al. Presence of one ecto- and two endoparasite species of the black stork (Ciconia nigra) in Portugal[J]. BMC Vet Res, 2021, 17(1): 21.
[3]	Heneberg P, Sitko J. Molecular phylogenetics provides unequivocal support for reclassifying Cathaemasia hians longivitellata and C. h. hians (Trematoda∶Cathaemasiidae) as two valid species with different host preferences[J]. J Helminthol, 2024, 98: e76.
[4]	Juhásová ?, K?nigová A, Molnár L, et al. First record of Cathaemasia hians (Trematoda∶Cathaemasiidae) in a new bird host, the eastern imperial eagle (Aquila heliaca)[J]. Helminthologia, 2023, 60(4): 380-384.
[5]	Rudolphi KA. Entozoorum sive vermium intestinalium historia naturalis[M]. Vol 2. Amsterdam: Tabernaelibrariae et artium, 1808: 457. (in Latin)
[6]	Looss A. Weitere Beitr?gezur Kenntniss der Trematoden-Fauna Aegyptens, zugleich Versuch einer natürlichen Gliederung des Genus Distomum Retzius[J]. Zool Jahrbücher, 1899, 12: 521-784. (in German)
[7]	赵世华, 杭福德, 常建华, 等. 黑鹳的呵欠鸟凯塞玛吸虫病[J]. 野生动物, 1990(2): 25.
	Zhao SH, Hang FD, Chang JH, et al. Black stork yawning bird kesema fluke disease[J]. Wildlife, 1990(2): 25. (in Chinese)
[8]	孔繁瑶. 家畜寄生虫学[M]. 2版. 北京: 中国农业大学出版社, 1997: 414-417
	Kong FY. Livestock parasitology[M]. 2nd ed. Beijing: China Agricultural University Press, 1997: 414-417. (in Chinese)
[9]	Goswami LM, Prasad PK, Tandon V, et al. Molecular characterization of Gastrodiscoides hominis (Platyhelminthes∶Trematoda∶Digenea) inferred from ITS rDNA sequence analysis[J]. Parasitol Res, 2009, 104(6): 1485-1490.
[10]	Yoshida S, Toyoda K. Notes on Cathaemasia hians (Rudolphi) from the mouth of Ciconia nigra[J]. Ann Trop Med Parasitol, 1930, 24(1): 85-94.
[11]	Liptovszky M, Majoros G, Perge E. Cathaemasia hians in a black stork (Ciconia nigra) in Hungary[J]. J Wildl Dis, 2012, 48(3): 809-811.
[12]	K?nigová A, Hr?ková G, Molnár L, et al. Cathaemasia hians infection in black stork in Slovakia: Morphological and histopathological study[J]. Helminthologia, 2015, 52(4): 316-322.
[13]	Merino S, Martinez J, Lanzarot P, et al. Cathaemasia hians (Trematoda∶Cathaemasiidae) infecting black stork nestlings (Ciconia nigra) from central Spain[J]. Avian Pathol, 2001, 30(5): 559-561.
[14]	Zhytova O, Korol E. Cathaemasia hians (Digenea, Cathaemasiidae) from Planorbis planorbis (Mollusca, Gastropoda) in reservoirs of central polissya[J]. Vestnik Zool, 2012, 46(4): 361-364.
[15]	Syrota YY, Kuzmin YI, Lyaskivskiy VN, et al. First record of Dicheilonema ciconiae (Nematoda, Diplotriaenoidea) from Ciconia nigra (Aves, Ciconiidae) in Ukraine[J]. Vestnik Zool, 2016, 50(4): 379-382.
[16]	Sitko J, Heneberg P. Composition, structure and pattern of helminth assemblages associated with central European storks (Ciconiidae)[J]. Parasitol Int, 2015, 64(2): 130-134.
[17]	Gonzálvez M, Mu?oz-Hernández C, Gómez de Ramón A, et al. Flying across Europe: The case of the spread of Chaunocephalus ferox on a black stork (Ciconia nigra)[J]. J Helminthol, 2022, 96: e80.
[8]	Caffara M, Locke SA, Gustinelli A, et al. Morphological and molecular differentiation of Clinostomum complanatum and Clinostomum marginatum (Digenea∶Clinostomidae) metacercariae and adults[J]. J Parasitol, 2011, 97(5): 884-891.

Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia

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