Prevalence and spatial distribution of Fasciola spp. in cattle in Fujian Province

doi:10.12140/j.issn.1000-7423.2025.04.021

Abstract

Abstract:

To investigate the prevalence and geographical distribution of Fasciola spp. in bovine in Fujian Province, 2 to 3 counties were selected from 9 cities and one comprehensive experimental area in Fujian Province from May 2023 to May 2024 as bovine stool sampling sites. Allocate sampling quantities based on the proportion of bovine in each county to the total number of bovine in the province, with one fecal sample taken from each bovine. The Fasciola eggs in bovine stool samples were collected using the precipitation method for morphological characterization. Ten Fasciola eggs were randomly sampled from positive fecal samples in each city (district), and genomic DNA was extracted from eggs for PCR amplification of ribosomal internal transcribed spacer region 2 (ITS2) gene. All statistical analyses were performed using the software SPSS 20.0. A total of 502 bovine stool samples were collected, and there 110 samples detected with Fasciola eggs, with 21.9% (110/502) prevalence of infections. The detected Fasciola eggs were elongated in shape, light yellow brown in color, and contained a small cap at one terminal. The Fasciola infection rates of the 10 cities (districts) from high to low are Pingtan District (52.8%, 19/36), Fuzhou City (42.5%, 34/80), Longyan City (37.9%, 25/66), Ningde City (23.5%, 8/34), Putian City (16.0%, 8/50), Zhangzhou City (12.8%, 6/47), Quanzhou City (10.3%, 6/58), Xiamen City (9.3%, 3/32), Sanming City (1.7%, 1/59), Nanping City (0/40), and there was a significant difference in the prevalence of Fasciola infections among the 10 cities (districts) (Fisher’s exact test, P < 0.01). Specific bands were amplified at 550 bp in Fasciola-infected bovine stool samples from 9 cities (districts). Spatial analysis showed that geographical distribution of Fasciola spp. was scattered in bovine in Fujian Province, with a high coverage rate and a landscape of “high in the northwestern region and low in the southeastern region”, with hotspots identified in Pingtan District, Fuzhou City, and Longyan City (prevalence of infection > 36.0% to 54.0%). The findings showed that the geographical distribution of Fasciola spp. is widespread in bovines in Fujian Province, with relatively high prevalence in livestock. There is a risk of infection with bovine fasciolosis among individuals living in Fujian Province.

Key words: Fasciola spp., Endemic area, Epidemiological characteristic, Investigation, Fujian

CLC Number:

R383.26

JIANG Dianwei, CAI Wuwei, ZHENG Dan, XIE Hanguo, GAO Lanlin. Prevalence and spatial distribution of Fasciola spp. in cattle in Fujian Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(4): 588-591.

Figures/Tables 2

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