关键词: 尖吻蝮蛇舌状虫, 若虫, 抗蠕虫药物, 治疗

Abstract: Objective To observe the effect of praziquantel， mebendazole， tribendimidine， ivermectin， artemether and dihydroartemisinin against Amillifer agkistrodontis nymphs harbored in mice. Method Thirty-five mice infected each with 40 eggs of Amillifer agkistrodontis for 25-37 weeks were divided into 10 groups （2-8 mice per group）. Among them, nine groups were treated orally with praziquantel 500 mg/（kg·d）×5 d or 500 mg/（kg·d）×14 d， mebendazole 300 mg/（kg·d）×5 d， tribendimidine 300 mg/（kg·d）×5 d， ivermectin 8-10 mg/（kg·d）×3 d or 15 mg/（kg·d）×14 d， artemether 400 mg/（kg·d）×5 d and dihydroartemisinin 200 mg/（kg·d）×5 d， respepctively. The remaining untreated group served as control. All mice were sacrificed 1-3 weeks post-treatment for collection and separation of Amillifer agkistrodontis nymph cysts from abdominal wall， lipid tissue and viscera including liver， spleen and outer wall of intestine. The nymphs were released after tearing up the cyst with forceps, placed in the normal saline to observe their motor activity and counted. Results The difference of mean nymph burden between the drug treated groups and control group was not statistically significant （P>0.05） with mean nymph reductions of 8.3%-35.0%. Meanwhile， the appearance of the cyst， the size， colour， morphology and motor activity of nymphs were also similar to that of the control. Conclusion Praziquantel，mebendazole，tribendimidine，ivermectin，artemether and dihydroartemisinin exhibit no effect against Amillifer agkistrodontis harbored in mice under the conditions in the experiment.

Key words: Armillifer agkistrodontis, Nymph, Anthelmintics, Treatment